Peptides pp 73-111 | Cite as

Immunocytochemical Techniques in Peptide Localization

Possibilities and Pitfalls
  • F. W. van Leeuwen
Part of the Neuromethods book series (NM, volume 6)

Abstract

During the past decade, immunocytochemistry (ICC) has been introduced into the neurosciences on a large scale. ICC has proved to be a powerful tool in supplementing and extending the data obtained with other techniques (e.g., electrophysiology, neuropharmacology, neurochemistry). Its strength is that it combines a high degree of specificity with the resolution of the light and electron microscope. Therefore it can be more or less regarded as “biochemistry within the tissue section,” and even more than that if the amount of immunoreactivity can be quantified. ICC alone also resulted in a large number of new data that changed a number of concepts (e.g., coexistence of peptides and amines, occurrence of hitherto assumed “organ- or tissue area-unique” peptides elsewhere; Hokfelt et al., 1980; Livett, 1978). At present, a wide variety of compounds can be localized by ICC (van Leeuwen et al., 1982). Here we will focus on peptide ICC, paying special attention to the pitfalls with which every immunocytochemist will be confronted as soon as the first successful localizations have been achieved. It will be illustrated that at present the technique to prove the specificity of antipeptide sera is still in its infancy.

Keywords

Epoxy Aldehyde Dehydration Catalase Gelatin 

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Copyright information

© The Humana Press Inc. 1987

Authors and Affiliations

  • F. W. van Leeuwen
    • 1
  1. 1.Netherlands lnstztute for Bram ResearchThe Netherlands

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