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Cell and Tissue Cultures

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General Neurochemical Techniques

Part of the book series: Neuromethods ((NM,volume 1))

Abstract

The methodology of tissue culture was introduced more than three quarters of a century ago by (1907) as “a method by which the end of a growing nerve could be brought under direct observation while alive, in order that a correct conception might be had regarding what lakes place as the fiber extends during embryonic developrnent from the nerve center out to the periphery” (Fig. 1). Thus, from the very beginning a major purpose of tissue culture studies has been to provide information about events occurring in vivo, and many of the early tissue culture studies were carried out to enable direct observations of living cells

Movements and changes in shape of the growing tip of a frog embryo nerve fiber during 47 min, at 4 d in culture. An erythrocyte serves as marker (after Harrison, 1907—from Murray 1965).

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Authors and Affiliations

  1. Department of Pharmacology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    L. Hertz

  2. Department of Anatomy, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    B. H. J. Juurlink

  3. Department of Neurology, University of Chicago, Chicago, Illinois

    S. Szuchet

  4. Department of Physiology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    W. Walz

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  1. L. Hertz
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  2. B. H. J. Juurlink
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  3. S. Szuchet
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  4. W. Walz
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Alan A. Boulton Glen B. Baker

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Hertz, L., Juurlink, B.H.J., Szuchet, S., Walz, W. (1985). Cell and Tissue Cultures. In: Boulton, A.A., Baker, G.B. (eds) General Neurochemical Techniques. Neuromethods, vol 1. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-075-x:117

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  • DOI: https://doi.org/10.1385/0-89603-075-x:117

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-075-6

  • Online ISBN: 978-1-59259-606-5

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