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Cell and Tissue Cultures

  • L. Hertz
  • B. H. J. Juurlink
  • S. Szuchet
  • W. Walz
Part of the Neuromethods book series (NM, volume 1)

Abstract

The methodology of tissue culture was introduced more than three quarters of a century ago by (1907) as “a method by which the end of a growing nerve could be brought under direct observation while alive, in order that a correct conception might be had regarding what lakes place as the fiber extends during embryonic developrnent from the nerve center out to the periphery” (Fig. 1). Thus, from the very beginning a major purpose of tissue culture studies has been to provide information about events occurring in vivo, and many of the early tissue culture studies were carried out to enable direct observations of living cells
Fig. 1.

Movements and changes in shape of the growing tip of a frog embryo nerve fiber during 47 min, at 4 d in culture. An erythrocyte serves as marker (after Harrison, 1907—from Murray 1965).

Keywords

Schwann Cell Explant Culture Cytosine Arabinoside Established Cell Line Glutamine Synthetase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Humana Press Inc. 1985

Authors and Affiliations

  • L. Hertz
    • 1
  • B. H. J. Juurlink
    • 2
  • S. Szuchet
    • 3
  • W. Walz
    • 4
  1. 1.Department of PharmacologyUniversity of SaskatchewanSaskatoonCanada
  2. 2.Department of AnatomyUniversity of SaskatchewanSaskatoonCanada
  3. 3.Department of NeurologyUniversity of ChicagoChicago
  4. 4.Department of PhysiologyUniversity of SaskatchewanSaskatoonCanada

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