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One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of 3H-Labeled RNA by Fluorography

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Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2))

Abstract

The procedure described in this chapter is used to display single-stranded nucleic acids according to their sizes, within the range of 0.5 to 30 kilobases (kb). Possible applications include examining products of in vitro synthesis, hybrid-selected RNAs from total cellular nucleic acids, and Northern blots. The method works equally well with RNA and DNA.

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References

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John M. Walker

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© 1984 The Humana Press Inc.

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Gurney, T. (1984). One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of 3H-Labeled RNA by Fluorography. In: Walker, J.M. (eds) Nucleic Acids. Methods in Molecular Biology, vol 2. Humana Press. https://doi.org/10.1385/0-89603-064-4:77

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  • DOI: https://doi.org/10.1385/0-89603-064-4:77

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-064-0

  • Online ISBN: 978-1-59259-489-4

  • eBook Packages: Springer Protocols

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