Abstract
Characterization of RNA molecules by electrophoresis or hybridization frequently requires nucleic acid concentrations over 1 mg/mL. High molecular weight DNA in a mixture of nucleic acids limits the solubility and interferes with electrophoresis. DNase treatment makes the mixture more soluble, even if DNA degradation is only partial.
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© 1984 The Humana Press Inc.
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Gurney, T., Gurney, E.G. (1984). Preparation of “RNase-Free” DNase by Alkylation. In: Walker, J.M. (eds) Nucleic Acids. Methods in Molecular Biology, vol 2. Humana Press. https://doi.org/10.1385/0-89603-064-4:13
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DOI: https://doi.org/10.1385/0-89603-064-4:13
Publisher Name: Humana Press
Print ISBN: 978-0-89603-064-0
Online ISBN: 978-1-59259-489-4
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