Abstract
The Burton assay for DNA is a colorimetric procedure for measuring the deoxyribose moiety of DNA. It is reasonably specific for deoxyribose, although very high concentrations of ribose (from RNA) or sucrose must be avoided. The method can be used on relatively crude extracts and in other circumstances where direct measurement of ultraviolet absorbance of denatured DNA is not practical. The assay has been widely used.
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References
Burton, K. (1956) A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of DNA. Biochem. J. 62, 315–323.
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© 1984 The Humana Press Inc.
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Waterborg, J.H., Matthews, H.R. (1984). The Burton Assay for DNA. In: Walker, J.M. (eds) Nucleic Acids. Methods in Molecular Biology, vol 2. Humana Press. https://doi.org/10.1385/0-89603-064-4:1
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DOI: https://doi.org/10.1385/0-89603-064-4:1
Publisher Name: Humana Press
Print ISBN: 978-0-89603-064-0
Online ISBN: 978-1-59259-489-4
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