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Nucleic Acids pp 293-300 | Cite as

Immunological Detection of Gene Expression in Recombinant Clones

  • J. W. Dale
  • P. J. Greenaway
Part of the Methods in Molecular Biology book series (MIMB, volume 2)

Abstract

After ligation and transformation, a number of clones will (it is hoped) be obtained that can be identified as recombinants by the occurrence of insertional inactivation, by analysis of small-scale plasmid preparations, or more specifically by in situ hybridization. An additional strategy that is superficially attractive, but not without considerable problems, is to look directly for expression of the cloned gene(s). This may be applicable in primary cloning if no specific DNA probe is available, but is more generally used in subsequent studies of expression of specific foreign genes.

Keywords

Millipore Filter Normal Rabbit Serum Plastic Disc Insertional Inactivation Primary Cloning 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Broome, S., and Gilbert, W. (1978) Immunological screening method to detect specific translation products. Proc. Natl. Acad. Sci. USA. 75, 2746–2749.PubMedCrossRefGoogle Scholar
  2. 2.
    Hudson, L., and Hay, F. C. (1980) Practical Immunology. Blackwell Scientific Publications, Oxford, UK.Google Scholar
  3. 3.
    Bolton, A. E. (1977) Radioiodination Techniques. Amersham International Ltd.Google Scholar
  4. 4.
    Clarke, L., Hitzeman, R., and Carbon, J. (1979) Selection of specific clones from colony banks by screening with radioactive antibody. Meth. Enzymology 68, 436–442.CrossRefGoogle Scholar
  5. 5.
    Erlich, H. A., Cohen, S. N., and McDevitt, H. O. (1979) Immunological detection and characterization of products translated from cloned DNA fragments. Meth. Enzymology 68, 443–453.CrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1984

Authors and Affiliations

  • J. W. Dale
    • 1
    • 2
  • P. J. Greenaway
    • 1
    • 2
  1. 1.Department of MicrobiologyUniversity of SurreyGuildford
  2. 2.Molecular Genetics LaboratoryPHLS Centre for Applied Microbiology and Research, Potion DownSalisburyUK

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