In Vitro Packaging of DNA
The E protein, which is the major component of the phage head; mutants that are defective in this gene are unable to assemble the preheads, and therefore accumulate the other, unassembled, components of the phage particle as well as the other proteins involved in packaging.
The D and A proteins, which are involved in the packaging process itself. Mutants that are defective in these genes are able to produce the preheads, but will not package DNA. This results in the accumulation of empty preheads.
KeywordsPhage Particle Packaging Extract Cosmid Vector Sonication Buffer Desired Size Range