Abstract
Thin-layer chromatography and electrophoresis, either separately or in combination, provide a simple, high resolution technique for producing peptide maps. Recently, thin-layer methods have tended to be increasingly overshadowed by the development of high performance liquid chromatography (HPLC). Although thin-layer methods are generally not as sensitive as HPLC (requiring milli- or microgram amounts of protein instead of the nanogram or less quantities used in HPLC), they are still preferred for many applications. Thin-layer peptide mapping not only has the advantage of being relatively inexpensive, but it is also a very simple technique and it is often possible to analyze a large number of different samples in a single experiment. In addition, a number of different stains can be used for the detection of specific amino acids and these considerably increase the value of this method.
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© 1984 Humana Press
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Gooderham, K. (1984). Peptide Mapping by Thin-Layer Chromatography and High Voltage Electrophoresis. In: Walker, J.M. (eds) Proteins. Methods in Molecular Biology™, vol 1. Humana Press. https://doi.org/10.1385/0-89603-062-8:179
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DOI: https://doi.org/10.1385/0-89603-062-8:179
Publisher Name: Humana Press
Print ISBN: 978-0-89603-062-6
Online ISBN: 978-1-59259-488-7
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