Part of the Methods in Molecular Biology™ book series (MIMB, volume 1)
Enzyme-Linked Immunosorbant Assay (ELISA)
In general, immunological methods are not very well suited for a quantitative determination of the antigen to be studied. The ELISA technique, however, can be used for a quantitative or at least semiquantitative determination of the concentration of a certain antigen. The method was first introduced by Engvall and Perlmann (1). The principle of ELISA (see Fig. 1), also called the double antibody sandwich technique, is the following: Antibodies against the antigen to be measured are adsorbed to a solid support, in most cases a polystyrene microtiter plate. After coating the support with antibody and washing, the antigen is added and will bind to the adsorbed antibodies. Next, a conjugate that will also bind to the antigen is added. Conjugates are antibody molecules to which an enzyme is covalently bound.
KeywordsSubstrate Solution Caprylic Acid Conjugate Solution Sodium Carbonate Buffer Polystyrene Microtiter Plate
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
© Humana Press 1984