Proteins pp 271-278 | Cite as

Immunofluorescence and Immunoperoxidase Screening of Hybridomas

  • J. N. Wood
Part of the Methods in Molecular Biology™ book series (MIMB, volume 1)


As mentioned in the previous chapter, the provision of a rapid, simple assay for screening monoclonal antibodies is essential for their production. Although solid-phase binding assays are the method of choice ( Chapter 30) a number of applications require visualization of antibody binding to cultured cells or tissue sections.


Formaldehyde H2O2 Sucrose Glycerol DMSO 


  1. 1.
    Lane, D. P., and Lane, E. B. (1981) A rapid antibody assay system for screening hybridoma cultures, J. Immunol. Methods 47, 303PubMedCrossRefGoogle Scholar
  2. 2.
    Sternberger, L. A. (1974) Immunocytochemistry, Prentice-Hall, Englewood Cliffs, NJ.Google Scholar

Copyright information

© Humana Press 1984

Authors and Affiliations

  • J. N. Wood
    • 1
  1. 1.Department of Experimental lmmunoblologyWellcome Research LaboratoriesBeckenham

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