Immunofluorescence and Immunoperoxidase Screening of Hybridomas
Part of the
Methods in Molecular Biology™
book series (MIMB, volume 1)
As mentioned in the previous chapter, the provision of a rapid, simple assay for screening monoclonal antibodies is essential for their production. Although solid-phase binding assays are the method of choice ( Chapter 30) a number of applications require visualization of antibody binding to cultured cells or tissue sections.
KeywordsAntibody Binding Sodium Borate Direct Conjugation Tissue Culture Fluid Monoclonal Antibody Binding
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Lane, D. P., and Lane, E. B. (1981) A rapid antibody assay system for screening hybridoma cultures, J. Immunol. Methods
, 303PubMedCrossRefGoogle Scholar
Sternberger, L. A. (1974) Immunocytochemistry
, Prentice-Hall, Englewood Cliffs, NJ.Google Scholar