Proteins pp 271-278 | Cite as

Immunofluorescence and Immunoperoxidase Screening of Hybridomas

  • J. N. Wood
Part of the Methods in Molecular Biology™ book series (MIMB, volume 1)

Abstract

As mentioned in the previous chapter, the provision of a rapid, simple assay for screening monoclonal antibodies is essential for their production. Although solid-phase binding assays are the method of choice ( Chapter 30) a number of applications require visualization of antibody binding to cultured cells or tissue sections.

Keywords

Formaldehyde H2O2 Sucrose Glycerol DMSO 

References

  1. 1.
    Lane, D. P., and Lane, E. B. (1981) A rapid antibody assay system for screening hybridoma cultures, J. Immunol. Methods 47, 303PubMedCrossRefGoogle Scholar
  2. 2.
    Sternberger, L. A. (1974) Immunocytochemistry, Prentice-Hall, Englewood Cliffs, NJ.Google Scholar

Copyright information

© Humana Press 1984

Authors and Affiliations

  • J. N. Wood
    • 1
  1. 1.Department of Experimental lmmunoblologyWellcome Research LaboratoriesBeckenham

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