Proteins pp 105-111 | Cite as

Isoelectric Focusing in Ultrathin Polyacrylamide Gels

  • Graham B. Divall
Part of the Methods in Molecular Biology™ book series (MIMB, volume 1)


Isoelectric focusing is an electrophoretic method for the separation of amphoteric macromolecules according to their isoelectric points (pI) in a stabilized natural pH gradient. It is a sensitive and reproducible technique, particularly valuable in the separation of closely related proteins that may not be easily separated by other techniques.


Bottom Plate Methylene Bisacrylamide Cooling Plate Carrier Ampholyte Glass Mold 
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    Randall, T., Harland, W. A., and Thorpe, J. W. (1980) A method of phenotyping erythrocyte acid phosphatase by isoelectric focusing. Med. Sci. Law 20, 43–47.PubMedGoogle Scholar
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    Divall, G. B. (1981) Studies on the use of isoelectric focusing as a method of phenotyping erythrocyte acid phosphatase. Forensic Sci. Int. 18, 67–78.PubMedCrossRefGoogle Scholar
  3. 3.
    Divall, G. B., and Ismail, M. (1983) Studies and observations on the use of isoelectric focusing in ultra-thin polyacrylamide gels as a method of typing human red cell phosphoglucomutase. Forensic Sci. Int. 22, 253–263.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press 1984

Authors and Affiliations

  • Graham B. Divall
    • 1
  1. 1.Metropolitan Police Forensic Science LaboratoryLondonEngland

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