Proteins pp 1-3 | Cite as

The Lowry Method for Protein Quantitation

  • Jaap H. Waterborg
  • Harry R. Matthews
Part of the Methods in Molecular Biology™ book series (MIMB, volume 1)

Abstract

The most accurate method of determining protein concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein and absolute concentrations cannot be obtained. The procedure of Lowry et al. (1) is no exception, but its sensitivity is moderately constant from protein to protein, and it has been so widely used that Lowry protein estimations are a completely acceptable alternative to a rigorous absolute determination in almost all circumstances where protein mixtures or crude extracts are involved.

Keywords

Vortex 5H2O Hydrolysis Albumin Boiling 

References

  1. 1.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.PubMedGoogle Scholar

Copyright information

© Humana Press 1984

Authors and Affiliations

  • Jaap H. Waterborg
    • 1
  • Harry R. Matthews
    • 1
  1. 1.Department of Biological Chemistry, School of MedicineUniversity of CaliforniaDavis

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