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Derivation, Maintenance, and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cells

  • Protocol
Embryonic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 329))

Abstract

We describe here the isolation and maintenance of pluripotent embryonic stem (ES) cells from equine blastocysts that have been frozen and thawed. Equine ES cells appear to main-tain a normal diploid karyotype in culture. These cells express markers that are characteris-tic of mouse ES cells, namely, alkaline phosphatase, stage-specific-embryonic antigen 1, STAT3, and Oct4. We also describe protocols for the induction of differentiation in vitro to neural precursor cells in the presence of basic fibroblast growth factor (bFGF), epidermal growth factor, and platelet-derived growth factor and to hematopoietic and endothelial cell lineages in the presence of bFGF, stem cell factor, and oncostatin M. Equine ES cells pro-vide a powerful tool for gene targeting and the generation of transgenic clonal offspring.

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Author information

Authors and Affiliations

  1. Saito Laboratory of Cell Technology, Kataoka, Yaita, Techigi, Japan

    Shigeo Saito

  2. Hokkaido Animal Research, Shintoku, Hokkaido, Japan

    Ken Sawai

  3. Hokkaido Animal Research, Shintoku, Hokkaido, Japan

    Arika Minamihashi

  4. Gene Engineering Division, BioResource, RIKEN (The Institute of Physical and Chemical Research), Ibaraki, Japan

    Hideyo Ugai

  5. Gene Engineering Division, BioResource Centre, RIKEN (The Institute of Physical and Chemical Research), Ibaraki, Japan

    Takehide Murata

  6. Gene Engineering Division, BioResource, RIKEN (The Institute of Physical and Chemical Research), Ibaraki, Japan

    Kazunari K. Yokoyama

Authors
  1. Shigeo Saito
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  2. Ken Sawai
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  3. Arika Minamihashi
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  4. Hideyo Ugai
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  5. Takehide Murata
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  6. Kazunari K. Yokoyama
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Editor information

Editors and Affiliations

  1. Ottawa Health Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2006 Humana Press Inc.

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Saito, S., Sawai, K., Minamihashi, A., Ugai, H., Murata, T., Yokoyama, K.K. (2006). Derivation, Maintenance, and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cells. In: Turksen, K. (eds) Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 329. Humana Press. https://doi.org/10.1385/1-59745-037-5:59

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  • DOI: https://doi.org/10.1385/1-59745-037-5:59

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-498-2

  • Online ISBN: 978-1-59745-037-9

  • eBook Packages: Springer Protocols

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