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Automated High-Throughput Protein Crystallization

  • Arezou Azarani
Protocol
  • 2.1k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

X-ray crystallography provides key biological insights into the three-dimensional structure and function of proteins, as well as essential information on protein-protein and protein-ligand interactions, therefore facilitating the design of more effective clinical drugs. The three most popular protein crystallization methods—vapor-diffusion sitting drop, hanging drop, and microbatch (1, 2, 3, 4, 5, 6), most commonly used by investigators-convey major economical disadvantages for the setup of rapid large-scale crystallization of new proteins. Lack of suitable automation for the numerous lengthy and labor-intensive setup steps, irreproducibility as a result of manual intervention, waste of precious and scarce protein samples caused by the absence of precise low-volume dispensers and appropriate plate technology, exorbitant consumption of time, and cost (4,5) are among the most common drawbacks of high-throughput crystallization.

Keywords

Protein Crystallization Crystallization Reaction Plate Technology Screen Plate Viscous Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Arezou Azarani
    • 1
  1. 1.Protogen ConsultingSan Jose

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