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Assessment of Antibody-Antigen Interaction Using SELDI Technology

  • Li-Shan Hsieh
  • Ramy Moharram
  • Emilia Caputo
  • Brian M. Martin
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The aim of this chapter is to discuss/describe both the theoretical aspects of the study of antibody-antigen interaction employing ProteinChip Array/sR technology, also known as surface-enhanced laser desorption/ionization-time-of-flight mass spectrom- etry (SELDI-TOF/TM MS), and to provide proven procedures for their use.

Keywords

Humid Chamber Chip Surface Sinapinic Acid Carbonyl Diimidazole ProteinChip Array 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Li-Shan Hsieh
    • 1
  • Ramy Moharram
    • 2
  • Emilia Caputo
    • 3
  • Brian M. Martin
    • 4
  1. 1.Division of New Drug Chemistry 1Center for Drug Evaluation and Research, Food and Drug AdministrationRockville
  2. 2.Laboratory of NeurotoxicologyNIMH, National Institutes of HealthBethesda
  3. 3.Institute of Genetics and BiophysicsNaplesItaly
  4. 4.Laboratory of NeurotoxicologyNIMH, National Institutes of HealthBethesda

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