Assessment of Antibody-Antigen Interaction Using SELDI Technology

  • Li-Shan Hsieh
  • Ramy Moharram
  • Emilia Caputo
  • Brian M. Martin
Part of the Springer Protocols Handbooks book series (SPH)


The aim of this chapter is to discuss/describe both the theoretical aspects of the study of antibody-antigen interaction employing ProteinChip Array/sR technology, also known as surface-enhanced laser desorption/ionization-time-of-flight mass spectrom- etry (SELDI-TOF/TM MS), and to provide proven procedures for their use.


Humid Chamber Chip Surface Sinapinic Acid Carbonyl Diimidazole ProteinChip Array 
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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Li-Shan Hsieh
    • 1
  • Ramy Moharram
    • 2
  • Emilia Caputo
    • 3
  • Brian M. Martin
    • 4
  1. 1.Division of New Drug Chemistry 1Center for Drug Evaluation and Research, Food and Drug AdministrationRockville
  2. 2.Laboratory of NeurotoxicologyNIMH, National Institutes of HealthBethesda
  3. 3.Institute of Genetics and BiophysicsNaplesItaly
  4. 4.Laboratory of NeurotoxicologyNIMH, National Institutes of HealthBethesda

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