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Quantitative Analysis of Protein Phosphorylation Status and Protein Kinase Activity on Microarrays Using Pro-Q− Diamond Dye Technology

  • Karen Martin
  • Wayne F. Patton
Protocol
  • 2.1k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The human genome is estimated to contain 30,000 to 75,000 genes, but as a result of alternative mRNA splicing and protein posttranslational modifications, the human proteome may contain a million or more proteins. Of the potential posttranslational modifications, phosphorylation by protein kinases is of increasing importance and interest because of its involvement in many essential cellular processes such as metabolism, transcription, cell-cycle regulation, and apoptosis, as well as intercellular communication. The malfunction of these and other kinase-regulated processes is often manifested in clinical disease states.

Keywords

Sodium Dodecyl Sulfate Alternative mRNA Splice Destain Solution Essential Cellular Process Microarray Staining 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Martin, K., Steinberg, T. H., Cooley, L. A., Gee, K. R., Beechem, J. M., and Patton, W. F. (2003) Quantitative analysis of protein phosphorylation status and protein kinase activity on microarrays using a novel fluorescent phosphorylation sensor dye. Proteomics 7, 244–255.Google Scholar
  2. 2.
    Steinberg, T. H., Agnew, B. J., Gee, K. R., et al. (2003) Global quantitative phosphopro-tein analysis using Multiplexed Proteomics technology. Proteomics 7, 1128–1144.CrossRefGoogle Scholar
  3. 3.
    Martin, K., Steinberg, T. H., Goodman, T., et al. (2003) Strategies and solid-phase formats for the analysis of protein and peptide phosphorylation employing a novel fluorescent phos-phorylation sensor dye. Comb. Chem. High Throughput Screen 4, 331–339.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Karen Martin
    • 1
  • Wayne F. Patton
    • 1
  1. 1.Molecular Probes Inc.Eugene

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