Quantitative Analysis of Protein Phosphorylation Status and Protein Kinase Activity on Microarrays Using Pro-Q− Diamond Dye Technology
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The human genome is estimated to contain 30,000 to 75,000 genes, but as a result of alternative mRNA splicing and protein posttranslational modifications, the human proteome may contain a million or more proteins. Of the potential posttranslational modifications, phosphorylation by protein kinases is of increasing importance and interest because of its involvement in many essential cellular processes such as metabolism, transcription, cell-cycle regulation, and apoptosis, as well as intercellular communication. The malfunction of these and other kinase-regulated processes is often manifested in clinical disease states.
KeywordsSodium Dodecyl Sulfate Alternative mRNA Splice Destain Solution Essential Cellular Process Microarray Staining
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