Peptide Sequences of 2-D Gel-Separated Protein Spots by Nanoelectrospray Tandem Mass Spectrometry
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This chapter describes the analysis of 2-D gel-separated protein spots by nanospray or nanoelectrospray tandem mass spectrometry (MS). The samples may also have originated from one-dimensional gel electrophoresis separations, or from micro- or nanobore multidimensional chromatography. The principles of the technique described below to obtain nanospray into an ion trap or into hybrid MS instruments are similar (1).