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Computer Analysis of 2-D Images

  • Patricia M. Palagi
  • Daniel Walther
  • Gérard Bouchet
  • Sonja Voordijk
  • Ron D. Appel
Protocol
  • 2.1k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Indeed, the development of two-dimensional (2-D) electrophoresis technology has provided an important research tool for comparative proteomics. A number of other technologies for protein separation have also been investigated in the last few years, such as protein chips, liquid chromatography, and high-pressure liquid chromatography, to name just a few. However, 2-D gels are still considered the workhorse among research laboratories willing to accurately track changes in protein expression and to achieve differential proteomics.

Keywords

Region Tool Characteristic Spot Important Research Tool Heuristic Cluster Paired Spot 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    Appel R. D., Vargas J. R., Palagi P. M., Walther D., and Hochstrasser D. F. (1997) Melanie II: a third generation software package for analysis of two-dimensional electrophoresis images-II. Algorithms. Electrophoresis 18, 2735–2748.Google Scholar
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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Patricia M. Palagi
    • 1
  • Daniel Walther
    • 2
  • Gérard Bouchet
    • 2
  • Sonja Voordijk
    • 3
  • Ron D. Appel
    • 4
  1. 1.Swiss Institute of BioinformaticsGeneva University HospitalGenevaSwitzerland
  2. 2.Swiss Institute of BioinformaticsGenevaSwitzerland
  3. 3.Geneva Bioinformatics S. A.GenevaSwitzerland
  4. 4.Swiss Institute of BioinformaticsUniversity and Geneva University HospitalGenevaSwitzerland

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