Zn2+Reverse Staining Technique

  • Carlos Fernandez-Patron
Part of the Springer Protocols Handbooks book series (SPH)


With the advent of proteomics, many previously unidentified proteins will be isolated in gels for subsequent structural and functional characterization. It is, therefore, important that methods are available for detecting these proteins with minimal risk of modification. This chapter describes a reverse staining technique that facilitates the sensitive detection of unmodified proteins (1, 2, 3, 4, 5, 6, 7, 8, 9).


Sodium Carbonate Native Page Coomassie Blue Zinc Sulfate Equilibration Step 
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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Carlos Fernandez-Patron
    • 1
  1. 1.Heart and Stroke Foundation New Investigator, Department of BiochemistryUniversity of AlbertaCanada

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