Preparation of Plant Protein Samples for 2-D PAGE
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A critical step in the application of O’Farrell’s (1) two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) in plant biology research is the preparation of plant protein samples free of artefactual protein modifications and without adversely affecting gel resolving power and reproducibility. However, it is important to note that many problems could be encountered in this step. A large part of the plant cell volume is often occupied by the vacuole. Numerous compounds found therein, upon release during disruption of plant cells, might have detrimental or interfering effects on protein extraction. These include organic acids, phenolic compounds, proteases, pigments, polysaccharides, and so on.
KeywordsPhenolic Compound Protamine Sulfate Page Analysis Pinus Radiata Hypocotyl Segment
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