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Preparation of Plant Protein Samples for 2-D PAGE

  • David W. M. Leung
Protocol
  • 2.2k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

A critical step in the application of O’Farrell’s (1) two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) in plant biology research is the preparation of plant protein samples free of artefactual protein modifications and without adversely affecting gel resolving power and reproducibility. However, it is important to note that many problems could be encountered in this step. A large part of the plant cell volume is often occupied by the vacuole. Numerous compounds found therein, upon release during disruption of plant cells, might have detrimental or interfering effects on protein extraction. These include organic acids, phenolic compounds, proteases, pigments, polysaccharides, and so on.

Keywords

Phenolic Compound Protamine Sulfate Page Analysis Pinus Radiata Hypocotyl Segment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • David W. M. Leung
    • 1
  1. 1.School of Biological Sciences, University of CanterburyNew Zealand

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