• Patricia A. Fetsch
Part of the Springer Protocols Handbooks book series (SPH)


Immunocytochemistry (aka immunohistochemistry), a technique used for the localization of specific cellular antigens, was introduced to diagnostic tumor pathology about 20 yr ago. The procedure allows for the visualization of antigens in tissue samples via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody, an enzyme complex, and a chromogenic substrate. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site, which is interpreted using a light microscope. Immunocytochemistry is routinely used in hospital laboratories to diagnose cancers, identify infectious organisms, differentiate malignant from benign processes, and reveal prognostic indicators.


Germ Cell Tumor Mantle Cell Lymphoma Islet Cell Tumor Prostatic Acid Phosphatase Nonseminomatous Germ Cell Tumor 
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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Patricia A. Fetsch
    • 1
  1. 1.Laboratory of PathologyNational Cancer Institute, National Institutes of HealthBethesda

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