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Immunocytochemistry

  • Patricia A. Fetsch
Protocol
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Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Immunocytochemistry (aka immunohistochemistry), a technique used for the localization of specific cellular antigens, was introduced to diagnostic tumor pathology about 20 yr ago. The procedure allows for the visualization of antigens in tissue samples via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody, an enzyme complex, and a chromogenic substrate. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site, which is interpreted using a light microscope. Immunocytochemistry is routinely used in hospital laboratories to diagnose cancers, identify infectious organisms, differentiate malignant from benign processes, and reveal prognostic indicators.

Keywords

Germ Cell Tumor Mantle Cell Lymphoma Islet Cell Tumor Prostatic Acid Phosphatase Nonseminomatous Germ Cell Tumor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Abati, A., Fetsch, P., and Filie, A. (1998) If cells could talk. The application of new techniques to cytopathology. Clin. Lab. Med. 18, 561–583.PubMedGoogle Scholar
  2. 2.
    Nadji, M., Ganjei, P., and Morales, A. (1994) Immunocytochemistry in contemporary cytology: the technique and its application. Lab. Med. 25, 502–508.Google Scholar
  3. 3.
    Leong, A. S-Y, Suthipintawong, C., and Vinyuvat, S. (1999) Immunostaining of cytologic preparations: a review of technical problems. Appl. Immunohistochem. 7, 214–220.CrossRefGoogle Scholar
  4. 4.
    Leung, S. W. and Bedard, Y. C. (1996) Immunocytochemical staining on ThinPrep processed smears. Mod. Pathol. 9, 304–306.PubMedGoogle Scholar
  5. 5.
    Fetsch, P.A., Simsir, A., Brosky, K., and Abati, A. (2002) Comparison of three commonly used cytologic preparations in effusion cytology. Diagn. Cytopathol. 26, 61–66.PubMedCrossRefGoogle Scholar
  6. 6.
    Han, A. C., Filstein, M. R., Hunt, J. V., Soler, A. P., Knudsen, K. A., and Salazar, H. (1999) Ncadherin distinguishes pleural mesotheliomas from lung adenocarcinomas: a ThinPrep immunocytochemical study. Cancer 87, 83–86.PubMedCrossRefGoogle Scholar
  7. 7.
    Cheepsumon, S., Leong, A. S.-Y., and Vinyuvat, S. (1996) Immunostaining of cell preparations: a comparative evaluation of common fixatives and protocols. Diagn. Cytopathol. 15, 167–174.CrossRefGoogle Scholar
  8. 8.
    Fan, Z., Clark, V., and Nagle, R. B. (1997) An evaluation of enzymatic and heat epitope retrieval methods for the immunohistochemical staining of the intermediate filaments. Appl. Immunohistochem. 5, 49–58.CrossRefGoogle Scholar
  9. 9.
    Shi, S.-R., Cote, R. J., Chaiwun, B., et al. (1998) Standardization of immunohistochemistry based on antigen retrieval technique for routine formalin-fixed tissue sections. Appl. Immunohistochem. 6, 89–96.CrossRefGoogle Scholar
  10. 10.
    Taylor, C. K., Shi, S.-R., and Cote, R. J. (1996) Antigen retrieval for immunohistochemistry: status and need for greater standardization. Appl. Immunohistochem. 4, 144–166.Google Scholar
  11. 11.
    Riera, J. R., Astengo-Osuna, C., Longmate, J. A., and Battifora, H. (1997) The immunohistochemical diagnostic panel for epithelial mesothelioma: a reevaluation after heat-induced epitope retrieval. Am. J. Surg. Pathol. 21, 1409–1419.PubMedCrossRefGoogle Scholar
  12. 12.
    Shi, S. R., Key, M. E., and Kalra, K. L. (1991) Antigen retrieval in formalin-fixed paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem. 39, 741–748.PubMedGoogle Scholar
  13. 13.
    Miller, R. T., Swanson, P. E., and Wick, M. R. (2000) Fixation and epitope retrieval in diagnostic immunohistochemistry: a concise review with practical considerations. Appl. Immunohistochem. Mol. Morphol. 8, 228–235.PubMedCrossRefGoogle Scholar
  14. 14.
    Hsu, S. M., Raine, L., and Fanger, H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 577–580.PubMedGoogle Scholar
  15. 15.
    Department of Health and Human Services, Health Care Financing Administration (1992) Clinical Laboratory Improvement Amendments of 1988; Final Rule. Fed. Reg. 57, 7001–7288.Google Scholar
  16. 17.
    Fetsch, P. A. and Abati, A. (1999) Overview of the clinical immunohistochemistry laboratory: regulations and troubleshooting guidelines, in Immunocytochemical Methods and Protocols (Javois, L. C., ed.), Humana, Totowa, NJ, pp. 405–414.CrossRefGoogle Scholar
  17. 18.
    O’Leary, T. J. (2001) Standardization in immunohistochemistry. Appl. Immunohistochem. Mol. Morphol. 9, 3–8.CrossRefGoogle Scholar
  18. 19.
    Taylor, C. R., Shi, S.-R., Barr, N. J., and Wu, N. (2001) Techniques of immunohistochemistry: principles, pitfalls and standardization, in Diagnostic Immunohistochemistry (Dabbs, D. J., ed), Churchill Livingstone, Philadelphia, PA, pp. 3–43.Google Scholar
  19. 20.
    Betta, P.-G., Andrion, A., Donna, A., et al. (1997) Malignant mesothelioma of the pleura: the reproducibility of the immunohistological diagnosis. Pathol. Res. Pract. 193, 759–765.PubMedGoogle Scholar
  20. 21.
    Shield, P. W., Callan, J. J., and Devine, P. L. (1994) Markers for metastatic adenocarcinoma in serous effusion specimens. Diagn. Cytopathol. 11, 237–245.PubMedCrossRefGoogle Scholar
  21. 22.
    Dejmek, A. and Hjerpe, A. (2000) Reactivity of six antibodies in effusions of mesothelioma, adenocarcinoma and mesotheliosis: stepwise logistic regression analysis. Cytopathology 11, 8–17.PubMedCrossRefGoogle Scholar
  22. 23.
    Dejmek, A. and Hjerpe, A. (1994) Immunohistochemical reactivity in mesothelioma and adenocarcinoma: a stepwise logistic regression analysis. APMIS 102, 255–264.PubMedCrossRefGoogle Scholar
  23. 24.
    Dejmek, A., Brockstedt, U., and Hjerpe, A. (1997) Optimization of a battery using nine immunocytochemical variables for distinguishing between epithelial mesothelioma and adenocarcinoma. APMIS 105, 889–894.PubMedCrossRefGoogle Scholar
  24. 25.
    Donna, A., Betta, P.-G., Bellingeri, D., Tallarida, F., Pavesi, M., and Pastormerlo, M. (1992) Cytologic diagnosis of malignant mesothelioma in serous effusions using an antimesothelial-cell antibody. Diagn. Cytopathol. 18, 361–365.CrossRefGoogle Scholar
  25. 26.
    Leong, A. and Vernon-Roberts, E. (1994) The immunohistochemistry of malignant mesothelioma. Pathol. Ann. 29(Pt. 2), 157–179.Google Scholar
  26. 27.
    Kitazume, H., Kitamura, K., Mukai, K., et al. (2000) Cytologic differential diagnosis among reactive mesothelial cells, malignant mesothelioma, and adenocarcinoma: utility of combined E-cadherin and calretinin immunostaining. Cancer 90, 55–60.PubMedCrossRefGoogle Scholar
  27. 28.
    Ordonez, N. (1998) Value of calretinin immunostaining in differentiating epithelial mesothelioma from lung adenocarcinoma. Mod. Pathol. 11, 929–933.PubMedGoogle Scholar
  28. 29.
    Ordonez, N. G. and Mackay, B. (1999) Glycogen-rich mesothelioma. Ultrastruct. Pathol. 23, 401–406.PubMedCrossRefGoogle Scholar
  29. 30.
    Oates, J. and Edwards, C. (2000) HBME-1, MOC-31, WT1, and calretinin: an assessment of recently described markers for mesothelioma and adenocarcinoma. Histopathology 36, 341–347.PubMedCrossRefGoogle Scholar
  30. 31.
    Cury, P. M., Butcher, D. N., Fisher, C., Corrin, B., and Nicholson, A. G. (2000) Value of the mesothelium-associated antibodies thrombomodulin, cytokeratin 5/6, calretinin, and CD44H in distinguishing epithelioid pleural mesothelioma from adenocarcinoma metastatic to the pleura. Mod. Pathol. 13, 107–112.PubMedCrossRefGoogle Scholar
  31. 32.
    Doglioni, C., Tos, A. P., Laurino, L., et al. (1996) Calretinin: a novel immunocytochemical marker for mesothelioma. Am. J. Surg. Pathol. 20, 1037–1046.PubMedCrossRefGoogle Scholar
  32. 33.
    Frisman, D. (2003) ImmunoQuery, an immunohistology query system. www.ipox.org.
  33. 34.
    Simsir, A., Fetsch, P. A., and Abati, A. (2001) Calretinin immunostaining in benign and malignant pleural effusions. Diagn. Cytopathol. 24, 149–152.PubMedCrossRefGoogle Scholar
  34. 35.
    Chhieng, D. C., Yee, H., Schaefer, D., et al. (2000) Calretinin staining pattern aids in the differentiation of mesothelioma from adenocarcinoma in serous effusions. Cancer 90, 194–200.PubMedCrossRefGoogle Scholar
  35. 36.
    Fetsch, P. A., Simsir, A., and Abati, A. (2001) Comparison of antibodies to HBME-1 and calretinin for the detection of mesothelial cells in effusion cytology. Diagn. Cytopathol. 25, 158–161.PubMedCrossRefGoogle Scholar
  36. 37.
    Wieczorek, T. J. and Krane, J. F. (2000) Diagnostic utility of calretinin immunohistochemistry in cytologic cell block preparations. Cancer 90, 312–319.PubMedCrossRefGoogle Scholar
  37. 38.
    Nagel, H., Hemmerlein, B., Ruschenburg, I., Huppe, K., and Droese, M. (1998) The value of anticalretinin antibody in the differential diagnosis of normal and reactive mesothelia versus metastatic tumors in effusion cytology. Pathol. Res. Pract. 194, 759–764.PubMedGoogle Scholar
  38. 39.
    Ordonez, N. G. (1989) The immunohistochemical diagnosis of mesothelioma: differentiation of mesothelioma and lung adenocarcinoma. Am. J. Surg. Pathol. 13, 276–291.PubMedGoogle Scholar
  39. 40.
    Cooper, D., Schermer, A., and Sun, T.-T. (1985) Biology of disease-classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations. Lab. Invest. 52, 243–256.PubMedGoogle Scholar
  40. 41.
    Moll, R., Franke, W., Schiller, D., Geiger, B., and Krepler, R. (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11–24.PubMedCrossRefGoogle Scholar
  41. 42.
    Chu, P., Wu, E., and Weiss, L. (2000) Cytokeratin 7 and cytokeratin 20 expression in epithelial neoplasms: a survey of 435 cases. Mod. Pathol. 13, 962–972.PubMedCrossRefGoogle Scholar
  42. 43.
    Lauritzen, A. F. (1989) Diagnostic value of monoclonal antibody B72.3 in detecting adenocarcinoma cells in serous effusions. APMIS 97, 761–766.PubMedCrossRefGoogle Scholar
  43. 44.
    Kho-Duffin, J., Tao, L.-C., Cramer, H., Catellier, M. J., Irons, D., and Ng, P. (1999) Cytologic diagnosis of malignant mesothelioma, with particular emphasis on the epithelial noncohesive cell type. Diagn. Cytopathol. 20, 57–62.PubMedCrossRefGoogle Scholar
  44. 45.
    Davidson, B., Risberg, B., Kristensen, G., et al. (1999) Detection of cancer cells in effusions from patients diagnosed with gynaecological malignancies: evaluation of five epithelial markers. Virchows Arch. 435, 43–39.PubMedCrossRefGoogle Scholar
  45. 46.
    Diaz-Arias, A. A., Loy, T. S., Bickel, J. T., and Chapman, R. K. (1993) Utility of BER-EP4 in the diagnosis of adenocarcinoma in effusions: an immunocytochemical study of 232 cases. Diagn. Cytopathol. 9, 516–521.PubMedCrossRefGoogle Scholar
  46. 47.
    Chenard-Neu, M. P., Kabou, A., Mechine, A., Brolly, F., Orion, B., and Bellocq, J. P. (1998) Immunohistochemistry in the differential diagnosis of mesothelioma and adenocarcinoma. Evaluation of 5 new antibodies and 6 traditional antibodies. Ann. Pathol. 18, 460–465.PubMedGoogle Scholar
  47. 48.
    Miedouge, M., Rouzaud, P., Salama, G., et al. (1999) Evaluation of seven tumour markers in pleural fluid for the diagnosis of malignant effusions. Br. J. Cancer 81, 1059–1065.PubMedCrossRefGoogle Scholar
  48. 49.
    Stoetzer, O. J., Munker, R., Darsow, M., and Wilmanns, W. (1999) P53-immunoreactive cells in benign and malignant effusions: diagnostic value using a panel of monoclonal antibodies and comparison with CEA-staining. Oncol. Rep. 6, 433–436.Google Scholar
  49. 50.
    Holzinger, A., Dingle, S., Bejarano, P. A., et al. (1996) Monoclonal antibody to thyroid transcription factor-1: production, characterization, and usefulness in tumor diagnosis. Hybridoma 15, 49–53.PubMedCrossRefGoogle Scholar
  50. 51.
    Ordonez, N. (2000) Value of thyroid transcription factor-1, E-cadherin, BG8, WT1, and CD44S immunostaining in distinguishing epithelial pleural mesothelioma from pulmonary and nonpulmonary adenocarcinoma. Am. J. Surg. Pathol. 24, 598–606.PubMedCrossRefGoogle Scholar
  51. 52.
    Sauvageot, J. and Epstein, J. I. (1998) Immunoreactivity for prostate-specific antigen and prostatic acid phosphatase in adenocarcinoma of the prostate: relation to progression following radical prostatectomy. Prostate 34, 29–33.PubMedCrossRefGoogle Scholar
  52. 53.
    Jacobs, I. and Bast R. C. (1989) The Ca 125 tumour associated antigen: a review of the literature. Hum. Reprod. 4, 1–12.PubMedGoogle Scholar
  53. 54.
    McCluggage, W. G. (2000) Recent advances in immunohistochemistry in the diagnosis of ovarian neoplasms. J. Clin. Pathol. 53, 327–334.PubMedCrossRefGoogle Scholar
  54. 55.
    Nap, M. (1998) Immunohistochemistry of CA 125. Unusual expression in normal tissues, distribution in the human fetus and questions around its application in diagnostic pathology. Int. J. Biol. Markers 13, 210–215.PubMedGoogle Scholar
  55. 56.
    Fishman, W. H., Inglis, I., Stolbach, L. L., and Krant, M. J. (1968) A serum alkaline phosphatase isoenzyme of human neoplastic cell origin. Cancer Res. 28, 150–154.PubMedGoogle Scholar
  56. 57.
    Manivel, J. C., Jessurun, J., Wick, M. R., and Dehner, L. P. (1987) Placental alkaline phosphatase immunoreactivity in testicular germ cell neoplasms. Am. J. Surg. Pathol. 11, 21–29.PubMedCrossRefGoogle Scholar
  57. 58.
    Koshida, K. and Wahren, B. (1990) Placental-like alkaline phosphatase in seminoma. Urol. Res. 18, 87–92.PubMedCrossRefGoogle Scholar
  58. 59.
    Javadpour, N. (1980) The role of biologic tumor markers in testicular cancer. Cancer 45(7 Suppl.), 1755–1761.PubMedGoogle Scholar
  59. 60.
    Bosl, G. J. and Chaganti, R. S. (1994) The use of tumor markers in germ cell malignancies. Hematol. Oncol. Clin. North Am. 8, 573–587.PubMedGoogle Scholar
  60. 61.
    Cochran, A. J. and Wen, D. R. (1985) S-100 protein as a marker for melanocytic and other tumours. Pathology 17, 340–345.PubMedCrossRefGoogle Scholar
  61. 62.
    Kahn, H. J., Marks, A., Thom, H., and Baumal, R. (1983) Role of antibody to S100 protein in diagnostic pathology. Am. J. Clin. Pathol. 79, 341–347.PubMedGoogle Scholar
  62. 63.
    Takahashi, K., Isobe, T., Ohtsuki, Y., Akagi, T., Sonobe, H., and Okuyama, T. (1984) Immunohistochemical study on the distribution of alpha and beta subunits of S-100 protein in human neoplasm and normal tissues. Virchows Arch. B. 45, 385–396.Google Scholar
  63. 64.
    Wick, M. R., Swanson, P. E., and Rocamora, A. (1988) Recognition of malignant melanoma by monoclonal antibody HMB-45. An immunohistochemical study of 200 paraffin-embedded cutaneous tumors. J. Cutan. Pathol. 15, 201–207.PubMedCrossRefGoogle Scholar
  64. 65.
    de Vries, T.J., Smeets, M., de Graaf, R., et al. (2001) Expression of gp100, MART-1, tyrosinase, and S100 in paraffin-embedded primary melanomas and locoregional, lymph node, and visceral metastases: implications for diagnosis and immunotherapy. A study conducted by the EORTC Melanoma Cooperative Group. J. Pathol. 193, 13–20.PubMedCrossRefGoogle Scholar
  65. 66.
    Boyle, J. L., Haupt, H. M., Stern, J. B., and Multhaupt, H. A. (2002) Tyrosinase expression in malignant melanoma, desmoplastic melanoma, and peripheral nerve tumors. Arch. Pathol. Lab. Med. 126, 816–22.PubMedGoogle Scholar
  66. 67.
    Orchard, G. E. (2000) Comparison of immunohistochemical labelling of melanocyte differentiation antibodies melan-A, tyrosinase and HMB 45 with NKIC3 and S100 protein in the evaluation of benign naevi and malignant melanoma. Histochem. J. 32, 475–481.PubMedCrossRefGoogle Scholar
  67. 68.
    Fetsch, P. A., Marincola, F. M., Filie, A., Hijazi, Y., Kleiner, D., and Abati, A. (1999) Melanomaassociated antigen recognized by T-cells (MART-1): the advent of a preferred immunocytochemical antibody for the diagnosis of metastatic malignant melanoma in fine needle aspirations. Cancer 87, 37–42.PubMedCrossRefGoogle Scholar
  68. 69.
    Kurtin, P. J. and Pinkus, G. S. (1985) Leukocyte common antigen-a diagnostic discriminant between hematopoietic and nonhematopoietic neoplasms in paraffin sections using monoclonal antibodies: correlation with immunologic studies and ultrastructural localization. Hum. Pathol. 16, 353–365.PubMedCrossRefGoogle Scholar
  69. 70.
    Brunning, R. D., Borowitz, M., Matutes, E., et al. (2001) Precursor B lymphoblastic leukaemia/ lymphoblastic lymphoma (precursor B-cell acute lymphoblastic leukaemia), in World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues (Jaffe, E. S., Harris, N. L., Stein, H., and Vardiman, J. W., eds.), IARC, Lyon, pp. 111–114.Google Scholar
  70. 71.
    Chu, P. G., Chang, K. L., Arber, D. A., and Weiss, L. M. (2000) Immunophenotyping of hematopoietic neoplasms. Semin. Diagn. Pathol. 17, 236–256.PubMedGoogle Scholar
  71. 72.
    Gatter, K. C. and Warnke, R. A. (2001) Diffuse large B-cell lymphoma, in World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues (Jaffe, E. S., Harris, N. L., Stein, H., and Vardiman, J. W., eds.), IARC, Lyon, pp. 171–174.Google Scholar
  72. 73.
    Mayall, F., Dray, M., Stanley, D., Harrison, B., and Allen R. (2000) Immunoflow cytometry and cell block immunohistochemistry in the FNA diagnosis of lymphoma: a review of 73 consecutive cases. J. Clin. Pathol. 53, 451-457.Google Scholar
  73. 74.
    Kikuchi, M., Jaffe, E. S., and Ralfkiaer, E. (2001) Adult T-cell leukaemia/lymphoma, in World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues (Jaffe, E. S., Harris, N. L., Stein, H., and Vardiman, J. W., eds.), IARC, Lyon, pp. 200–203.Google Scholar
  74. 75.
    Stein, H., Delsol, G., Pileri, S., et al. (2001) Classical Hodgkin lymphoma, in World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues (Jaffe, E. S., Harris, N. L., Stein, H., and Vardiman, J. W., eds.), IARC, Lyon, pp. 244–253.Google Scholar
  75. 76.
    Chittal, S. M., Caveriviere, P., Schwarting, R., et al. (1988) Monoclonal antibodies in the diagnosis of Hodgkin’s disease. The search for a rational panel. Am. J. Clin. Pathol. 12, 9–21.Google Scholar
  76. 77.
    Arber, D. A. and Weiss, L. M. (1993) CD15: a review. Appl. Immunohistochem. 1, 17–30.Google Scholar
  77. 78.
    Cras, P., Federsppiel, S. S., Gheuens, J., Martin, J. J., and Lowenthal, A. (1986) Demonstration of neuron-specific enolase in nonneuronal tumors using a specific monoclonal antibody. Ann. Neurol. 20, 106–107.PubMedCrossRefGoogle Scholar
  78. 79.
    Cras, P., Martin, J. J., and Gheuens, J. (1988) Gamma-enolase and glial fibrillary acidic protein in nervous system tumors. An immunohistochemical study using specific monoclonal antibodies. Acta Neuropathol. 75, 377–384.PubMedCrossRefGoogle Scholar
  79. 80.
    Wilson, B. S. and Lloyd, R. V. (1984) Detection of chromogranin in neuroendocrine cells with a monoclonal antibody. Am. J. Pathol. 115, 458–468.PubMedGoogle Scholar
  80. 81.
    Wiedenmann, B., Kuhn, C., Schwechheimer, K., et al. (1987) Synaptophysin identified in metastases of neuroendocrine tumors by immunocytochemistry and immunoblotting. Am. J. Clin. Pathol. 88, 560–569.PubMedGoogle Scholar
  81. 82.
    Wiedenmann, B. and Franke, W. W. (1985) Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell 41, 1017–1028.PubMedCrossRefGoogle Scholar
  82. 83.
    Al-Khafaji, B., Noffsinger, A. E., Miller, M. A., DeVoe, G., Stemmermann, G. N., and Fenoglio-Preiser, C. (1998) Immunohistologic analysis of gastrointestinal and pulmonary carcinoid tumors. Hum. Pathol. 29, 992–999.PubMedCrossRefGoogle Scholar
  83. 84.
    Goldstein, N. S. and Silverman, J. F. (2001) Immunohistochemistry of the gastrointestinal tract, pancreas, bile ducts, gallbladder and liver, in Diagnostic Immunohistochemistry (Dabbs, D. J., ed.), Churchill Livingstone, Philadelphia, pp. 333–406.Google Scholar
  84. 85.
    Azumi, N. and Battifora, H. (1987) The distribution of vimentin and keratin in epithelial and nonepithelial neoplasms. Am. J. Clin. Pathol. 88, 286–296.PubMedGoogle Scholar
  85. 86.
    Skalli, O., Gabbiani, G., Babai, F., Seemayer, T. A., Pizzolato, G., and Schurch, W. (1988) Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. II. Rhabdomyosarcomas. Am. J. Pathol. 130, 515–531.PubMedGoogle Scholar
  86. 87.
    Ohsawa, M., Naka, N., Tomita, Y., Kawamori, D., Kanno, H., and Aozasa, K. (1995) Use of immunohistochemical procedures in diagnosing angiosarcoma. Evaluation of 98 cases. Cancer 75, 2867–2874.PubMedCrossRefGoogle Scholar
  87. 88.
    Fellinger, E. J., Garin-Chesa, P., Su, S. L., DeAngelis, P., Lane, J. M., and Rettig, W. J. (1991) Immunohistochemical analysis of Ewing’s sarcoma cell surface antigen p30/32MIC2. Am. J. Surg. Pathol. 139, 317–325.Google Scholar
  88. 89.
    Ozdemirli, M., Fanburg-Smith, J. C., Hartmann, D. P., Azumi, N., and Miettinen, M. (2001) Differentiating lymphoblastic lymphoma and Ewing’s sarcoma: lymphocyte markers and gene rearrangement. Mod. Pathol. 14, 1175–1182.PubMedCrossRefGoogle Scholar
  89. 90.
    Pritchard, K. I. (2003) Endocrine therapy of advanced disease: analysis and implications of the existing data. Clin. Cancer Res. 9, 460–467.Google Scholar
  90. 91.
    McGuire, W. L., Carbone, P. P., Sears, M. E., and Escher, G. C. (1975) Estrogen receptors in human breast cancer: an overview, in Estrogen Receptors in Human Breast Cancer (McGuire, W. L., Carbone, P. P., and Vollmer, E. P., eds.), Raven, New York, pp. 1–7.Google Scholar
  91. 92.
    Slamon, D. J., Leyland-Jones, B., Shak, S., et al. (2001) Use of chemotherapy plus a monoclonal antibody against HER2 for metastatic breast cancer that overexpresses HER2. N. Engl. J. Med. 344, 783–792.PubMedCrossRefGoogle Scholar
  92. 93.
    Pegram, M. D., Lipton, A., Hayes, D. F., et al. (1998) Phase II study of receptor-enhanced chemosensitivity using recombinant humanized anti-p185HER2/neu monoclonal antibody plus cisplatin in patients with HER2/neu-overexpressing metastatic breast cancer refractory to chemotherapy treatment. J. Clin. Oncol. 16, 2659–2671.PubMedGoogle Scholar
  93. 94.
    Gancberg, D., Lespagnard, L., Rouas, G., et al. (2000) Sensitivity of HER-2/neu antibodies in archival tissue samples of invasive breast carcinomas. Correlation with oncogene amplification in 160 cases. Am. J. Clin. Pathol. 113, 675–682.PubMedCrossRefGoogle Scholar
  94. 95.
    Heinrich, M. C., Griffith, D. J., Druker, B. J., Wait, C. L., Ott, K. A., and Zigler, A. J. (2000) Inhibition of c-kit receptor tyrosine kinase activity by STI 571, a selective tyrosine kinase inhibitor. Blood 96, 925–932.PubMedGoogle Scholar
  95. 96.
    Demetri, G. D., von Mehren, M., Blanke, C. D., et al. (2002) Efficacy and safety of imatinib mesylate in advanced gastrointestinal stromal tumors. N. Engl. J. Med. 347, 472–480.PubMedCrossRefGoogle Scholar
  96. 97.
    Mauro, M. J. and Druker, B. J. (2001) STI571: targeting BCR-ABL as therapy for CML. Oncologist 6, 233–238.PubMedCrossRefGoogle Scholar
  97. 98.
    Hernandez-Boluda, J. C. and Cervantes, F. (2002) Imatinib mesylate (Gleevec, Glivec): a new therapy for chronic myeloid leukemia and other malignancies. Drugs Today (Barc.) 38, 601–613.CrossRefGoogle Scholar
  98. 99.
    Weiss, L. M., Movahed, L. A., Warnke, R. A., and Sklar, J. (1989) Detection of Epstein-Barr viral genomes in Reed-Sternberg cells of Hodgkin’s disease. N. Engl. J. Med. 320, 502–506.PubMedCrossRefGoogle Scholar
  99. 100.
    Mathur, V. S., Olson, J. L., Darragh, T. M., and Yen, T. S. (1997) Polyomavirus-induced interstitial nephritis in two renal transplant recipients: case reports and review of the literature. Am. J. Kidney Dis. 29, 754–758.PubMedCrossRefGoogle Scholar
  100. 101.
    Pappo, O., Demetris, A. J., Raikow, R. B., and Randhawa, P. S. (1996) Human polyoma virus infection of renal allografts: histopathologic diagnosis, clinical significance and literature review. Mod. Pathol. 9, 105–109.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Patricia A. Fetsch
    • 1
  1. 1.Laboratory of PathologyNational Cancer Institute, National Institutes of HealthBethesda

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