Abstract
This chapter is devoted to a simple method for culturing developing rat cerebellum. The method can also be used for rat hippocampus embryonic age 18 d (E18), cerebral cortex (E18), and even spinal cord, but at an earlier stage of development (E14). This method was initially designed to obtain long-term survival of cerebellar macroneurons, Purkinje cells, and deep nuclear macroneurons (Moonen et al, 1982; Neale et al., 1982). Indeed, in cultures in which a single cell suspension is seeded, virtually all the neurons die between 5 and 10 d, but significant survival is obtained if small aggregates of cells (microexplants), rather than single cells, are seeded. The term “microexplant” was used to stress the difference from the classic cerebellar explants, which consist of a thin cerebellar slice, which are much more organized tissue samples.
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Further Reading
Cull-Candy, S. G. and Usowicz, M. M. (1987), Multiple-conductance channels activated by excitatory amino acids in cerebellar neurons. Nature 325, 525–528.
Eagle, H. (1959), Amino acid metabolism in mammalian cell cultures. Science 130, 432–437.
Fischer, G., Kunemund, V., and Schachner, M. (1986), Neurite outgrowth patterns in cerebellar microexplant cultures are affected by antibodies to the cell surface glycoprotein L1. J. Neurosci. 6, 605–612.
Gibbs, W., Neale, E. A., and Moonen, G. (1982), Kainic acid sensitivity of mammalian Purkinje cells in monolayer cultures. Dev. Brain Res. 4, 103–108.
Grau-Wagemans, M. P., Selak, I., Lefebvre, P. P., and Moonen, G. (1984), Cerebellar macroneurons in serum-free cultures: evidence for intrinsec neuronotrophic and neuronotoxic activities. Dev. Brain Res. 15, 11–19.
Magyar-Lehmann, S., Suter, C. S., Stahel, W., and Schachner, M. (1995), Behaviour of small inhibitory interneurons in early postnatal mouse cerebellar microexplant cultures: a video time-lapse analysis. Eur. J. Neurosci, 7, 1449–1459.
Magyar-Lehmann, S., Frei, T., and Schachner, M. (1995b), Fasciculation of granule cell neurites is responsible for the perpendicular orientation of small inhibitory interneurons in mouse cerebellar microexplant cultures in vitro. Eur. J. Neurosci. 7, 1460–1471.
McDonald, R. L., Moonen, G., Neale, E. A., and Nelson, P. G. (1982), Cerebellar macroneurons in microexplant cell culture. Postsynaptic amino acid pharmacology. Dev. Brain Res. 5, 77–88.
Moonen, G., Neale, E. A., McDonald, R. L., Gibbs, W., and Nelson, P. G. (1982), Cerebellar macroneurons in microexplant cell culture. Methodology, basic electrophysiology and morphology after horseradish peroxidase injection. Dev. Brain Res. 5, 59–73.
Neale, E. A., Moonen, G., McDonald, R. L., and Nelson, P. G. (1982), Cerebellar macroneurons in microexplant cell culture: ultrastructural morphology. Neuroscience 7, 1879–1890.
Schmidt, C., Kunemund, V., Wintergerst, E. S., Schmitz, B., and Schachner, M. (1996), CD9 of mouse brain is implicated in neurite outgrowth with the alpha 6/beta 1 integrin and the neural adhesion molecule L1. J. Neurosci. Res. 43, 12–31.
Schmidt, C., Ohlmeyer, C., Labrakakis, C., Walter, T., Kettenmann, H., and Schnitzer, J. (1997), Analysis of motile oligodendrocyte precursor cells in vitro and in brain slices. Glia 20, 284–298.
Selak, I., Foidart, J. M., and Moonen, G. (1985), Laminin promotes cerebellar granule cell migration in vitro and is synthetized by cultured astrocytes. Dev. Neurosci. 7, 278–285.
Zempel, J. M. and Steinbach J. H. (1995), Neonatal rat cerebellar granule and Purkinje neurons in culture express different GABAA receptors. Eur. J. Neurosc. 7, 1895–1905.
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© 2001 Humana Press Inc., Totowa, NJ
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Rogister, B., Moonen, G. (2001). Microexplant Cultures of the Cerebellum. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-207-4:39
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DOI: https://doi.org/10.1385/1-59259-207-4:39
Publisher Name: Humana Press
Print ISBN: 978-0-89603-902-5
Online ISBN: 978-1-59259-207-4
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