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Microexplant Cultures of the Cerebellum

  • Bernard Rogister
  • Gustave Moonen
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

This chapter is devoted to a simple method for culturing developing rat cerebellum. The method can also be used for rat hippocampus embryonic age 18 d (E18), cerebral cortex (E18), and even spinal cord, but at an earlier stage of development (E14). This method was initially designed to obtain long-term survival of cerebellar macroneurons, Purkinje cells, and deep nuclear macroneurons (Moonen et al, 1982; Neale et al., 1982). Indeed, in cultures in which a single cell suspension is seeded, virtually all the neurons die between 5 and 10 d, but significant survival is obtained if small aggregates of cells (microexplants), rather than single cells, are seeded. The term “microexplant” was used to stress the difference from the classic cerebellar explants, which consist of a thin cerebellar slice, which are much more organized tissue samples.

Keywords

Purkinje Cell Erlenmeyer Flask Neuritic Outgrowth Plastic Petri Dish Outward Migration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Further Reading

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Bernard Rogister
    • 1
  • Gustave Moonen
    • 2
  1. 1.Research Center for Cellular and Molecular NeuroscienceInstitut Lèon FrédéricqLiegeBelgium
  2. 2.Department of NeurologyUniversity of Liege and Research Center for Cellular and Molecular Neuroscience, Institut Lèon FrédéricqLiegeBelgium

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