Quantification of Cells in Culture

  • Arleen Richardson
  • Sergey Fedoroff
Part of the Springer Protocols Handbooks book series (SPH)


Cell enumeration using the hemocytometer is applicable when determining the number of cells in a suspension, and when the number of samples to be analyzed is relatively small. Hemocytometry is also useful for determining the proportion of singly dispersed cells in a suspension, and for estimating the frequency of viable cells.


Counting Chamber Tritiated Thymidine Multiwell Plate Nonviable Cell Scintillation Solution 
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Further Reading

  1. Bainbridge, D. R. and Macey, M. M. (1983), Hoechst 33258: a fluorescent nuclear counterstain suitable for double-labelling immunofluorescence. J. Immunol. Methods 62, 193–195.PubMedCrossRefGoogle Scholar
  2. Baserga, R. (1989), Measuring parameters of growth, in Cell Growth and Division, Baserga, R., ed., IRL, New York.Google Scholar
  3. Branch, D. R. and Guilbert, L. J. (1986), Practical in vitro assay systems for the measurement of hematopoietic growth factors J. Tissue Culture Methods 10, 101–108.CrossRefGoogle Scholar
  4. Hamilton, L. H. (1956), Errors in blood cell counting. 1. Technical errors. 2. Statistical errors. Can. J. Med. Tech. 18, 8–14.Google Scholar
  5. Hanks, J. H. and Wallace, R. E. (1958), Determination of cell viability. Proc. Soc. Exp. Biol Med. 98, 188–192.PubMedGoogle Scholar
  6. Harris, M. (1959), Growth measurements on monolayer cultures with an electronic cell counter. Can. Res. 19, 1020–1024.Google Scholar
  7. Jones, K. H. and Senft, J. A. (1985), An improved method to determine cell viability by simultaneous staining with fluorescein diacetate propidium iodide. J. Hist. Cytol. 33, 77–79.Google Scholar
  8. Kaltenbach, J. P., Kaltenbach, M. H., and Lyons, W. B. (1958), Nigrosin as a dye for differentiating live and dead ascites cells. Exp. Cell Res. 15, 112–117.PubMedCrossRefGoogle Scholar
  9. Latt, S. A. and Stetten, G. (1976), Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. J. Histochem. Cytochem. 24, 24–33.PubMedGoogle Scholar
  10. Phillips, H. J. and Terryberry, J. E. (1957), Counting actively metabolizing tissue cultured cells. Exp. Cell Res. 13, 341–347.PubMedCrossRefGoogle Scholar
  11. Schrek, R. (1944), Studies in vitro on the physiology of normal and of cancerous cells. II. The survival and the glycolysis of cells under anaerobic conditions. Arch. Pathol. 37, 319–327.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Arleen Richardson
    • 1
  • Sergey Fedoroff
    • 1
  1. 1.Department of Anatomy and Cell Biology, College of MedicineUniversity of SaskatchewanSaskatoonCanada

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