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Colony Cultures

Plating Efficiency Assay and Cloning
  • Sergey Fedoroff
  • Arleen Richardson
Protocol
  • 2.8k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The premise of colony culturing is the assumption that single viable cells can attach to the substratum, divide, and form a progeny of cells that constitute a cell colony or clone. Colony cultures can be initiated either from a disaggregated cell suspension made directly from animal tissue, or from primary or secondary cultures or cell lines. Colonies, especially ones initiated directly from tissues, are not all identical. They vary in the morphology of their cells, and in their size and compactness. The morphology and size of the colony depends on the kinds of cells plated, interactions between cells, the degree of their differentiation, the cell generation time, the composition of the medium, the type of substratum, and the physical conditions to which the cells are subjected.

Keywords

Cell Coloni Culture Vessel Plate Efficiency Limit Dilution Method Mouse Neopallium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Further Reading

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Sergey Fedoroff
    • 1
  • Arleen Richardson
    • 1
  1. 1.Department of Anatomy and Cell Biology, College of MedicineUniversity of SaskatchewanSaskatoonCanada

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