Colony Cultures

Plating Efficiency Assay and Cloning
  • Sergey Fedoroff
  • Arleen Richardson
Part of the Springer Protocols Handbooks book series (SPH)


The premise of colony culturing is the assumption that single viable cells can attach to the substratum, divide, and form a progeny of cells that constitute a cell colony or clone. Colony cultures can be initiated either from a disaggregated cell suspension made directly from animal tissue, or from primary or secondary cultures or cell lines. Colonies, especially ones initiated directly from tissues, are not all identical. They vary in the morphology of their cells, and in their size and compactness. The morphology and size of the colony depends on the kinds of cells plated, interactions between cells, the degree of their differentiation, the cell generation time, the composition of the medium, the type of substratum, and the physical conditions to which the cells are subjected.


Cell Coloni Culture Vessel Plate Efficiency Limit Dilution Method Mouse Neopallium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Further Reading

  1. Brunette, D. M., Melcher, A. H., and Moe, H. K. (1976), Culture and origin of epithelium-like and fibroblast-like cells from porcine periodontal ligament explants and cell suspensions. Arch. Oral Biol. 21, 393–400.PubMedCrossRefGoogle Scholar
  2. Blakley, P. M. and Fedoroff, S. (1985), Effects of alcohol exposure on growth and cerebral function in DBA/1J mice. Int. J. Dev. Neurosci. 3, 69–76.CrossRefGoogle Scholar
  3. Cannow, T. B., Barbarese, E., and Carson, J. H. (1991), Diversification of glial lineages: a novel method to clone brain cells in vitro on nitrocellulose substratum. Glia 4, 256–268.CrossRefGoogle Scholar
  4. Clarke, J., Thorpe, R., and Davis, J. (1994), Cloning, in: Basic Cell Culture. A Practical Approach., Davis, J. M., ed., Oxford University Press, Oxford, UK.Google Scholar
  5. Fedoroff, S. (1984), A method for the study of neural cell lineages based on colony culture and transplantation of cultured cells into the CNS, in: Developmental Neuroscience: Physiological, Pharmacological and Clinical Aspects, Caciagle, F., Giacobini, E., Paoletti, R., eds., Elsevier, Amsterdam, pp. 373–376.Google Scholar
  6. Fedoroff, S. and Doering, L. C. (1980), Colony culture of neural cells as a method for the study of cell lineages in the developing CNS: the astrocyte cell lineage. Curr. Topics Dev. Biol. 16, 283–304.CrossRefGoogle Scholar
  7. Fedoroff, S., Zhai, R., and Novak, P. J. (1997), Microglia and astroglia have a common progenitor cell. J. Neurosci. Res. 50, 477–486.PubMedCrossRefGoogle Scholar
  8. Ham, R. G. (1972), Cloning of mammalian cells. Methods Cell Physiol. 5, 37–74.CrossRefGoogle Scholar
  9. Lefkovits, I. and Waldmann, H. (1979), Limiting Dilution Analysis of Cells in the Immune System. Cambridge University Press, Cambridge, UK.Google Scholar
  10. Moore, S. C., McCormack, J. M., Armendariz, E., Gatewood, J., and Walker, W. S. (1992), Phenotypes and alloantigen-presenting activity of individual clones of microglia derived from the mouse brain. J. Neuroimmunol. 41, 203–214.PubMedCrossRefGoogle Scholar
  11. Puck, T. T., Marcus, P. I., and Cieciura, S. J. (1956), Clonal growth of mammalian cells in vitro: growth characteristics of colonies from single HeLa cells with and without a “feeder” layer. J. Exp. Med. 103, 273–284.PubMedCrossRefGoogle Scholar
  12. Temple, S. (1989), Division and differentiation of isolated CNS blast cells in microculture. Nature 340, 471–473.PubMedCrossRefGoogle Scholar
  13. Temple, S. and Raff, M. C. (1985), Differentiation of a bipotential glial progenitor cell in single cell microculture. Nature 313, 223–225.PubMedCrossRefGoogle Scholar
  14. Temple, S. and Raff, M. C. (1986), Clonal analysis of oligodendrocyte development in culture: evidence for a developmental clock that counts cell divisions. Cell 44, 773–779.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Sergey Fedoroff
    • 1
  • Arleen Richardson
    • 1
  1. 1.Department of Anatomy and Cell Biology, College of MedicineUniversity of SaskatchewanSaskatoonCanada

Personalised recommendations