Neural Cell Counting

Cell Number Determination in Microplate Cultures
  • Marston Manthorpe
Part of the Springer Protocols Handbooks book series (SPH)


The purpose of this chapter is to introduce the reader to standard procedures for quantifying the number of neural cells in microcultures. The chick embryo was chosen as the source of neural cells, because embryos can be conveniently grown in an inexpensive laboratory incubator, in large, replicate numbers with defined developmental stages. Also, chicken embryos are used by many investigators as a routine source of peripheral and central nervous system tissue. Nevertheless, the procedure outlined can be adapted to neural cells from any source.


Chick Embryo Pasteur Pipet Optic Lobe Plastic Petri Dish Central Nervous System Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Further Reading

  1. Eagle, H. (1955), Nutrition needs of mammalian cells in tissue culture. Science 122, 501–504.PubMedCrossRefGoogle Scholar
  2. Geldof, A. A., Mastbergen, S. C., Henrar, R. E., and Faircloth, G. T. (1999), Cytotoxicity and neurocytotoxicity of new marine anticancer agents evaluated using in vitro assays. Cancer Chemother Pharmacol. 44, 312–318.PubMedCrossRefGoogle Scholar
  3. Gerlier, D. and Thomasset, N. (1986), Use of MTT colorimetric assay to measure cell activation. J. Immunol. Methods 94, 57–63.PubMedCrossRefGoogle Scholar
  4. Hamburger, V. and Hamilton, H. L. (1951), A series of normal stages in the development of the chick embryo. J. Morphol 88, 49–92.CrossRefGoogle Scholar
  5. Jost, L. M., Kirkwood, J. M., and Whiteside, T. L. (1992), Improved short-and long-term XTT-based colorimetric cellular cytotoxicity assay for melanoma and other tumor cells. J. Immunol. Methods 147, 153–165.PubMedGoogle Scholar
  6. Kobari, M., Kullenberg, B., Bjorkman, A., Matsuno, S., Ihse, I., and Axelson, J. (1998), The inhibitory effect of an EGF receptor-specific tyrosine kinase inhibitor on pancreatic cancer cell lines was more potent than inhibitory antibodies against the receptors for EGF and IGF I. Int. J. Pancreatol. 2, 85–95.Google Scholar
  7. Kondo, T., Wada, K., Kawashima, M., Sato, Y., and Yamauchi, M. (1994), High-sensitivity antitumor drug sensitivity testing. Oncology 5, 535–539.CrossRefGoogle Scholar
  8. Manthorpe, M., Skaper, S. D., and Varon, S. (1981), Neuronotrophic factors and their antibodies: in vitro microassays for titration and screening. Brain Res. 230, 295–306.PubMedCrossRefGoogle Scholar
  9. Manthorpe, M., Fagnani, R., Skaper, S. D., and Varon, S. (1986), An automated colorimetric microassay for neuronotrophic factors. Dev. Br. Res. 25, 191–198.CrossRefGoogle Scholar
  10. Mosmann, T. (1983), Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J. Immunol. Methods 65, 55–63.PubMedCrossRefGoogle Scholar
  11. Roehm, N. W., Rodgers, G. H., Hatfield, S. M., and Glasebrook A. L. (1991), An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. J. Immunol. Methods 142, 257–265.PubMedCrossRefGoogle Scholar
  12. Varon, S., Skaper, S. D., Barbin, G., Selak, I., and Manthorpe, M. (1984), Low molecular weight agents support survival of cultured neurons from the CNS. J. Neurosci. 4, 654–658.PubMedGoogle Scholar
  13. Weislow, O. S., Kiser, R., Fine, D. L., Bader, J., Shoemaker, R. H., and Boyd, M. R. (1989), New soluble-formazan assay for HIV-1 cytopathic effects: application to high-flux screening of synthetic and natural products for AIDS-antiviral activity. J. Natl. Cancer Inst. 81, 577–586. Published erratum appears in J. Natl. Cancer Inst. 81, 963.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Marston Manthorpe
    • 1
  1. 1.Vical Inc.San Diego

Personalised recommendations