Neural Cell Counting
- 2.8k Downloads
The purpose of this chapter is to introduce the reader to standard procedures for quantifying the number of neural cells in microcultures. The chick embryo was chosen as the source of neural cells, because embryos can be conveniently grown in an inexpensive laboratory incubator, in large, replicate numbers with defined developmental stages. Also, chicken embryos are used by many investigators as a routine source of peripheral and central nervous system tissue. Nevertheless, the procedure outlined can be adapted to neural cells from any source.
KeywordsChick Embryo Pasteur Pipet Optic Lobe Plastic Petri Dish Central Nervous System Tissue
- Kobari, M., Kullenberg, B., Bjorkman, A., Matsuno, S., Ihse, I., and Axelson, J. (1998), The inhibitory effect of an EGF receptor-specific tyrosine kinase inhibitor on pancreatic cancer cell lines was more potent than inhibitory antibodies against the receptors for EGF and IGF I. Int. J. Pancreatol. 2, 85–95.Google Scholar
- Weislow, O. S., Kiser, R., Fine, D. L., Bader, J., Shoemaker, R. H., and Boyd, M. R. (1989), New soluble-formazan assay for HIV-1 cytopathic effects: application to high-flux screening of synthetic and natural products for AIDS-antiviral activity. J. Natl. Cancer Inst. 81, 577–586. Published erratum appears in J. Natl. Cancer Inst. 81, 963.PubMedCrossRefGoogle Scholar