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Neural Cell Counting

Cell Number Determination in Microplate Cultures
  • Marston Manthorpe
Protocol
  • 2.8k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The purpose of this chapter is to introduce the reader to standard procedures for quantifying the number of neural cells in microcultures. The chick embryo was chosen as the source of neural cells, because embryos can be conveniently grown in an inexpensive laboratory incubator, in large, replicate numbers with defined developmental stages. Also, chicken embryos are used by many investigators as a routine source of peripheral and central nervous system tissue. Nevertheless, the procedure outlined can be adapted to neural cells from any source.

Keywords

Chick Embryo Pasteur Pipet Optic Lobe Plastic Petri Dish Central Nervous System Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Marston Manthorpe
    • 1
  1. 1.Vical Inc.San Diego

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