Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures

  • Ruth Cole
  • Jean de Vellis
Part of the Springer Protocols Handbooks book series (SPH)


The study of glial cell development and function has been considerably enhanced by the development of methods to culture oligodendrocytes and astrocytes from central nervous system tissue. A primary mixed glial culture, composed mostly of astrocytes, oligodendrocytes, and microglia, is obtained when newborn disaggregated cerebral brain cells from rat are plated at high cell density (2 × 105/cm2) in serum-supplemented medium (McCarthy and de Vellis, 1980).


Glial Fibrillary Acidic Protein Cerebral Hemisphere Central Nervous System Tissue Olfactory Lobe Median Fissure 
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Further Reading

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Ruth Cole
    • 1
  • Jean de Vellis
    • 1
  1. 1.Neuropsychiatric InstituteUniversity of California at Los AngelesLos Angeles

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