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Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures

  • Ruth Cole
  • Jean de Vellis
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The study of glial cell development and function has been considerably enhanced by the development of methods to culture oligodendrocytes and astrocytes from central nervous system tissue. A primary mixed glial culture, composed mostly of astrocytes, oligodendrocytes, and microglia, is obtained when newborn disaggregated cerebral brain cells from rat are plated at high cell density (2 × 105/cm2) in serum-supplemented medium (McCarthy and de Vellis, 1980).

Keywords

Glial Fibrillary Acidic Protein Cerebral Hemisphere Central Nervous System Tissue Olfactory Lobe Median Fissure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Further Reading

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Ruth Cole
    • 1
  • Jean de Vellis
    • 1
  1. 1.Neuropsychiatric InstituteUniversity of California at Los AngelesLos Angeles

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