Purification of IgG by Precipitation with Polyethylene Glycol (PEG)

  • Mark Page
  • Robin Thorpe
Part of the Springer Protocols Handbooks book series (SPH)


PEG precipitation works well for IgM, but is less efficient for IgG; salt precipitation methods are usually recommended for IgG. PEG precipitation may be preferred in multistep purifications that use ion-exchange columns, because the ionic strength of the Ig is not altered. Furthermore, it is a very mild procedure that usually results in little denaturation of antibody. This procedure is applicable to both polyclonal antisera and most MAb containing fluids.


Ionic Strength Ammonium Sulfate Polyethylene Glycol Precipitation Method Sulfate Precipitation 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Mark Page
    • 1
  • Robin Thorpe
    • 2
  1. 1.Apovia Inc.San Diego
  2. 2.Division of ImmunobiologyNational Institute for Biological Standards and ControlPotters BarUK

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