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Purification of IgG by Precipitation with Sodium Sulfate or Ammonium Sulfate

  • Mark Page
  • Robin Thorpe
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Addition of appropriate amounts of salts, such as ammonium or sodium sulfate, causes precipitation of IgG (1) from all mammals, and can be used for serum, plasma, ascites fluid, and hybridoma culture supernatant. Although such IgG is usually contaminated with other proteins, the ease of these precipitation procedures coupled with the high yield of IgG has led to their wide use in producing enriched IgG preparations. They are suitable for many immunochemical procedures, e.g., production of immunoaffinity columns, and as a starting point for further purification. It is not suitable however for conjugating with radiolabels, enzymes, or biotin since the contaminating proteins will also be conjugated, thereby reducing the efficiency of the labeling and the quality of the reagent. The precipitated IgG is usually very stable, and such preparations are ideally suited for long-term storage or distribution and exchange between laboratories.

Keywords

Sodium Sulfate Ammonium Sulfate Ascites Fluid Ammonium Sulfate Precipitation Saturated Ammonium Sulfate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Heide, K. and Schwick, H. G. (1978) Salt fractionation of immunoglobulins, in Handbook of Experimental Immunology, 3rd ed. (Weir, D. M., ed.),  Chap. 7. Blackwell Scientific, Oxford, UK.Google Scholar
  2. 2.
    Neoh, S. H., Gordon, C., A., and Zola, H. (1986) The purification of mouse MAb from ascitic fluid. J. Immunol. Meth. 91, 231.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Mark Page
    • 1
  • Robin Thorpe
    • 2
  1. 1.Apovia Inc.San Diego
  2. 2.Division of ImmunobiologyNational Institute for Biological Standards and ControlPotters BarUK

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