Purification and Assessment of Quality of Radioiodinated Protein
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At the end of a radioiodination procedure, the reaction mixture will contain the labeled protein, unlabeled protein, radioiodide, mineral salts, enzyme (in the case of the lactoperoxidase method), and possibly some protein that has been damaged during the oxidation. For most uses of radioiodinated proteins, it is essential to have the labeled species as pure as possible. In theory, any of the many methods of purifying proteins can be employed (1). However, the purification of the radioiodinated protein should be achieved as rapidly as possible. For that purpose, the most widely used of all separation techniques is gel filtration.