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Affinity Chromatography of Oligosaccharides and Glycopeptides with Immobilized Lectins

  • Kazuo Yamamoto
  • Tsutomu Tsuji
  • Toshiaki Osawa
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Sugar moieties on the cell surface play one of the most important roles in cellular recognition. To elucidate the molecular mechanism of these cellular phenomena, assessment of the structure of sugar chains is indispensable. However, it is difficult to elucidate the structures of cell surface oligosaccharides owing to two technical problems. The first is the difficulty in fractionating various oligosaccharides heterogeneous in the number, type, and substitution patterns of outer sugar branches. The second problem is that very limited amounts of material can be available, which makes it difficult to perform detailed structural studies. Lectins are proteins with sugar binding activity. Each lectin binds specifically to a certain sugar sequence in oligosaccharides and glycopeptides. To overcome these problems, lectins can serve as very useful tools. Recently, many attempts have been made to fractionate oligosaccharides and glycopeptides on immobilized lectin columns. The use of a series of immobilized lectin columns, whose sugar binding specificities have been precisely elucidated, enables us to fractionate a very small amount of radioactive oligosaccharides or glycopeptides (∼10 ng depending on the specific activity) into structurally distinct groups. In this chapter, we summarize the serial lectin-Sepharose affinity chromatographic technique for rapid, sensitive, and specific fractionation and analysis of asparagine-linked oligosaccharides of glycoproteins.

Keywords

Sialic Acid Complex Type Column Volume Sugar Chain High Mannose Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Kazuo Yamamoto
    • 1
  • Tsutomu Tsuji
    • 2
  • Toshiaki Osawa
    • 3
  1. 1.Department of Integrated Biosciences, Graduate School of Frontier SciencesUniversity of TokyoJapan
  2. 2.Hoshi Pharmaceutical CollegeTokyoJapan
  3. 3.Yakult Central Institute for Microbiology ResearchTokyoJapan

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