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Sialic Acid Analysis by HPAEC-PAD

  • Elizabeth F. Hounsell
  • Michael J. Davies
  • Kevin D. Smith
Protocol
  • 131 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The most labile monosaccharides are the family of sialic acids, which are usually chain-terminating substituents. These are therefore usually released first by either mild acid hydrolysis or enzyme digestion, and can be analyzed with great sensitivity by high pH anion-exchange chromatography with pulsed amperometric detection (HPAECPAD [1,2]). The remaining oligosaccharide is analyzed as discussed in  Chapter 111 to identify the position of linkage of the sialic acid.

Keywords

Sialic Acid Newcastle Disease Virus Newcastle Disease Chromic Acid Pulse Amperometric Detection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Townsend, R. R. (1995) Analysis of glycoconjugates using high-pH anion-exchange chromatography. J. Chromatog. Library 58, 181–209.CrossRefGoogle Scholar
  2. 2.
    Manzi, A. E., Diaz, S., and Varki, A. (1990) HPLC of sialic acids on a pellicular resin anion exchange column with pulsed amperometry. Anal. Biochem. 188, 20–32.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Elizabeth F. Hounsell
    • 1
  • Michael J. Davies
  • Kevin D. Smith
  1. 1.School of Biological and Chemical SciencesBirkbeck University of LondonUK

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