Sialic Acid Analysis by HPAEC-PAD

  • Elizabeth F. Hounsell
  • Michael J. Davies
  • Kevin D. Smith
Part of the Springer Protocols Handbooks book series (SPH)


The most labile monosaccharides are the family of sialic acids, which are usually chain-terminating substituents. These are therefore usually released first by either mild acid hydrolysis or enzyme digestion, and can be analyzed with great sensitivity by high pH anion-exchange chromatography with pulsed amperometric detection (HPAECPAD [1,2]). The remaining oligosaccharide is analyzed as discussed in  Chapter 111 to identify the position of linkage of the sialic acid.


Sialic Acid Newcastle Disease Virus Newcastle Disease Chromic Acid Pulse Amperometric Detection 
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  1. 1.
    Townsend, R. R. (1995) Analysis of glycoconjugates using high-pH anion-exchange chromatography. J. Chromatog. Library 58, 181–209.CrossRefGoogle Scholar
  2. 2.
    Manzi, A. E., Diaz, S., and Varki, A. (1990) HPLC of sialic acids on a pellicular resin anion exchange column with pulsed amperometry. Anal. Biochem. 188, 20–32.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Elizabeth F. Hounsell
    • 1
  • Michael J. Davies
  • Kevin D. Smith
  1. 1.School of Biological and Chemical SciencesBirkbeck University of LondonUK

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