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Determination of Monosaccharide Linkage and Substitution Patterns by GC-MS Methylation Analysis

  • Elizabeth F. Hounsell
  • Michael J. Davies
  • Kevin D. Smith
Protocol
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Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The GC or GC-MS method discussed in  Chapter 110 can distinguish substituted monosaccharides, but to characterize the position of acyl groups together with the linkages between the monosaccharides, a strategy has been developed to “capture” the substitution pattern by methylation of all free hydroxyl groups. The constituent monosaccharides are then analyzed after hydrolysis, reduction, and acetylation as partially methylated alditol acetates in a procedure known as methylation analysis (1-3).

Keywords

Methylation Analysis Methyl Iodide Substitution Pattern Constituent Monosaccharide Acetamido Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Hansson, G. C. and Karlsson, H. (1993) Gas chromatography and gas chromatographymass spectrometry of glycoprotein oligosaccharides, in Methods in Molecular Biology, vol. 14: Glycoprotein Analysis in Biomedicine (Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 47–54.CrossRefGoogle Scholar
  2. 2.
    Güther, M. L. and Ferguson, M. A. J. (1993) The microanalysis of glycosyl phosphatidylinositol glycans, in Methods in Molecular Biology, vol. 14: Glycoprotein Analysis in Biomedicine(Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 99–117.CrossRefGoogle Scholar
  3. 3.
    Hounsell, E. F. (1994) Physicochemical analysis of oligosaccharide determinants of glycoproteins.Adv. Carbohyd. Chem. Biochem. 50, 311–350.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Elizabeth F. Hounsell
    • 1
  • Michael J. Davies
  • Kevin D. Smith
  1. 1.School of Biological and Chemical SciencesBirkbeck University of LondonUK

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