Monosaccharide Analysis by Gas Chromatography (GC)
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Although the methods given in Chapter 109 can give an approximate idea of oligosaccharide amount or composition, they would not be able to distinguish the multiple monosaccharides and substituents present in nature. For this, the high resolution of gas chromatography (GC) is required (1,2). The most unambiguous results are provided by analysis of trimethylsilyl ethers (TMS) of methyl glycosides with on line mass selective detection (MS).
KeywordsSialic Acid Phosphorus Pentoxide Acetic Anhydride Acetyl Chloride Trimethylsilyl Ether