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Monosaccharide Analysis by Gas Chromatography (GC)

  • Elizabeth F. Hounsell
  • Michael J. Davies
  • Kevin D. Smith
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Although the methods given in  Chapter 109 can give an approximate idea of oligosaccharide amount or composition, they would not be able to distinguish the multiple monosaccharides and substituents present in nature. For this, the high resolution of gas chromatography (GC) is required (1,2). The most unambiguous results are provided by analysis of trimethylsilyl ethers (TMS) of methyl glycosides with on line mass selective detection (MS).

Keywords

Sialic Acid Phosphorus Pentoxide Acetic Anhydride Acetyl Chloride Trimethylsilyl Ether 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Hounsell, E. F. (1993) A general strategy for glycoprotein oligosaccharide analysis, in Methods in Molecular Biology, vol. 14: Glycoprotein Analysis in Biomedicine (Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 1–15.CrossRefGoogle Scholar
  2. 2.
    Hounsell, E. F. (1994) Physicochemical analyses of oligosaccharide determinants of glycoproteins. Adv. Carbohyd. Chem. Biochem. 50, 311–350.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Elizabeth F. Hounsell
    • 1
  • Michael J. Davies
  • Kevin D. Smith
  1. 1.School of Biological and Chemical SciencesBirkbeck University of LondonUK

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