Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin Blotting

  • Patricia Gravel
Part of the Springer Protocols Handbooks book series (SPH)


Glycoproteins result from the covalent association of carbohydrate moieties (gly-cans) with proteins. The enzymatic glycosylation of proteins is a common and complex form of posttranslational modification. The precise roles played by the carbohydrate moieties of glycoproteins are beginning to be understood (1-3). It has been established that glycans perform important biological roles including: stabilization of the protein structure; protection from degradation; and control of protein solubility, protein transport in cells, and protein half-life in blood. They also mediate the interactions with other macromolecules and the recognition and association with viruses, enzymes, and lectins (4-6).


Carbohydrate Moiety Colorimetric Reaction Neuraminic Acid Carbohydrate Binding Protein Important Biological Role 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Patricia Gravel
    • 1
  1. 1.Triskel Integrated ServicesGenevaSwitzerland

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