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Detection of Glycoproteins in Gels and Blots

  • Nicolle H. Packer
  • Malcolm S. Ball
  • Peter L. Devine
  • Wayne F. Patton
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

As we become more aware of the significance of posttranslational modifications, such as glycosylation, in the production of recombinant proteins and in the proteomic studies of development and disease, techniques for the identification and characterization of the oligosaccharides attached to proteins need to be established.

Keywords

Sodium Dodecyl Sulfate Acid Hydrazide Monosaccharide Composition Alkaline Phosphate Neuraminic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Gander J. E. (1984) Gel protein stains: glycoproteins. Meth. Enzymol. 104, 447–451.PubMedCrossRefGoogle Scholar
  2. 2.
    Packer, N. H., Wilkins, M. R., Golaz, O., Lawson, M. A., Gooley, A. A., Hochstrasser, D. F., et al. (1996) Characterization of human plasma glycoproteins separated by two-dimensional gel electrophoresis. Bio. Technol. 14, 66–70Google Scholar
  3. 3.
    Thornton, D. J., Carlstedt, I., and Sheehan, J. K. (1996) Identification of Glycoproteins on Nitrocellulose Membranes and Gels,Mol. Biotechnol. 5, 171–176.PubMedCrossRefGoogle Scholar
  4. 4.
    Devine, P. L. and Warren, J. A. (1990) Glycoprotein detection on Immobilon PVDF transfer membrane using the periodic acid/Schiff reagent,BioTechniques 8, 492–495.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Nicolle H. Packer
    • 1
  • Malcolm S. Ball
    • 2
  • Peter L. Devine
    • 3
  • Wayne F. Patton
    • 4
  1. 1.Proteome Systems Ltd.SydneyAustralia
  2. 2.Co-operative Research Centre for Eye Research TechnologySydneyAustralia
  3. 3.Proteome Systems Ltd.SydneyAustralia
  4. 4.Molecular Probes Inc.Eugene

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