Isolation of Proteins Cross-linked to DNA by Formaldehyde
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Formaldehyde is a reversible cross-linker that will cross-link protein to DNA, RNA, or protein (1). Because of its high-resolution (2 Å) cross-linking, formaldehyde is a useful agent to cross-link a DNA binding protein of interest to DNA. For example, formaldehyde has been used to cross-link proteins to DNA in studies fine-mapping the distribution of particular DNA binding proteins along specific DNA sequences (1,2).
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