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Isolation of Proteins Cross-linked to DNA by Formaldehyde

  • Virginia A. Spencer
  • James R. Davie
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Formaldehyde is a reversible cross-linker that will cross-link protein to DNA, RNA, or protein (1). Because of its high-resolution (2 Å) cross-linking, formaldehyde is a useful agent to cross-link a DNA binding protein of interest to DNA. For example, formaldehyde has been used to cross-link proteins to DNA in studies fine-mapping the distribution of particular DNA binding proteins along specific DNA sequences (1,2).

Keywords

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These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Orlando, V. (2000) Mapping chromosomal proteins by in vivo formaldehyde-crosslinked-chromatin immunoprecipitation. Trends Biochem. Sci. 25, 99–104.PubMedCrossRefGoogle Scholar
  2. 2.
    Dedon, P. C., Soults, J. A., Allis, C. D., and Gorovsky, M. A. (1991) A simplified formal-dehyde fixation and immunoprecipitation technique for studying protein-DNA interactions. Analyt. Biochem. 197, 83–90.PubMedCrossRefGoogle Scholar
  3. 3.
    Orlando, V., Strutt H., and Paro, R. (1997) Analysis of chromatin structure by in vivo formaldehyde crosslinking. Methods 11, 205–214.PubMedCrossRefGoogle Scholar
  4. 4.
    Kadosh, D. and Struhl, K. (1998) Targeted recruitment of the Sin3-Rpd3 histone deacetylase complex generates a highly localized domain of repressed chromatin in vivo. Mol. Cell. Biol. 18, 5121–5127.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Virginia A. Spencer
    • 1
  • James R. Davie
    • 2
  1. 1.Manitoba Institute of Cell BiologyManitobaCanada
  2. 2.Manitoba Institute of Cell BiologyWinnipegCanada

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