Isolation of Proteins Cross-linked to DNA by Formaldehyde

  • Virginia A. Spencer
  • James R. Davie
Part of the Springer Protocols Handbooks book series (SPH)


Formaldehyde is a reversible cross-linker that will cross-link protein to DNA, RNA, or protein (1). Because of its high-resolution (2 Å) cross-linking, formaldehyde is a useful agent to cross-link a DNA binding protein of interest to DNA. For example, formaldehyde has been used to cross-link proteins to DNA in studies fine-mapping the distribution of particular DNA binding proteins along specific DNA sequences (1,2).


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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Virginia A. Spencer
    • 1
  • James R. Davie
    • 2
  1. 1.Manitoba Institute of Cell BiologyManitobaCanada
  2. 2.Manitoba Institute of Cell BiologyWinnipegCanada

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