Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins

  • John M. Walker
Part of the Springer Protocols Handbooks book series (SPH)


The preparation of fixed-concentration polyacrylamide gels has been described in  Chapters 10 and  11. However, the use of polyacrylamide gels that have a gradient of increasing acrylamide concentration (and hence decreasing pore size) can sometimes have advantages over fixed-concentration acrylamide gels. During electrophoresis in gradient gels, proteins migrate until the decreasing pore size impedes further progress. Once the ”pore limit“ is reached, the protein banding pattern does not change appreciably with time, although migration does not cease completely (1). There are two main advantages of gradient gels over linear gels.


Ammonium Persulfate Pump Speed Decrease Pore Size Protein Banding Pattern Acrylamide Solution 
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  1. 1.
    Margolis, J. and Kenrick, K. G. (1967) Nature (London), 214, 1334.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • John M. Walker
    • 1
  1. 1.Department of BiosciencesUniversity of Hertfordshire, School of Natural SciencesHatfieldUK

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