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Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins

  • John M. Walker
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The preparation of fixed-concentration polyacrylamide gels has been described in  Chapters 10 and  11. However, the use of polyacrylamide gels that have a gradient of increasing acrylamide concentration (and hence decreasing pore size) can sometimes have advantages over fixed-concentration acrylamide gels. During electrophoresis in gradient gels, proteins migrate until the decreasing pore size impedes further progress. Once the ”pore limit“ is reached, the protein banding pattern does not change appreciably with time, although migration does not cease completely (1). There are two main advantages of gradient gels over linear gels.

Keywords

Ammonium Persulfate Pump Speed Decrease Pore Size Protein Banding Pattern Acrylamide Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Reference

  1. 1.
    Margolis, J. and Kenrick, K. G. (1967) Nature (London), 214, 1334.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • John M. Walker
    • 1
  1. 1.Department of BiosciencesUniversity of Hertfordshire, School of Natural SciencesHatfieldUK

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