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2-D Phosphopeptide Mapping

  • Hikaru Nagahara
  • Robert R. Latek
  • Sergei A. Ezhevsky
  • Steven F. Dowdy
Protocol
  • 205 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

A major mechanism that cells use to regulate protein function is by phosphorylation and/or dephosphorylation of serine, threonine, and tyrosine residues. Phosphopeptide mapping of these phosphorylated residues allows investigation into the positive and negative regulatory roles these sites may play in vivo. In addition, phosphopeptide mapping can uncover the specific phosphorylated residue and, hence, kinase recognition sites, thus helping in the identification of the relevant kinase(s) and/or phosphatase(s).

Keywords

Performic Acid Cooling Plate Regulate Protein Function MWCO Dialysis Tubing Supernatant Waste 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Hikaru Nagahara
    • 1
  • Robert R. Latek
    • 1
  • Sergei A. Ezhevsky
    • 1
  • Steven F. Dowdy
    • 1
  1. 1.Howard Hughes Medical InstituteWashington University School of MedicineSt. Louis

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