Detection of Disulfide-Linked Peptides by HPLC

  • Alastair Aitken
  • Michèle Learmonth
Part of the Springer Protocols Handbooks book series (SPH)


Classical techniques for determining disulfide bond patterns usually require the fragmentation of proteins into peptides under low pH conditions to prevent disulfide exchange. Pepsin or cyanogen bromide are particularly useful (see  Chapters 76 and  71 respectively).


Cyanogen Bromide Iodoacetic Acid Peptide Bond Cleavage Reverse Phase HPLC Column Reductive Alkylation 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Alastair Aitken
    • 1
  • Michèle Learmonth
    • 2
  1. 1.Division of Biomedical and Clinical Laboratory Sciences, Membrane Biology GroupUniversity of EdinburghScotland, UK
  2. 2.Department of Biomedical SciencesUniversity of EdinburghScotland

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