Detection of Disulfide-Linked Peptides by HPLC
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Classical techniques for determining disulfide bond patterns usually require the fragmentation of proteins into peptides under low pH conditions to prevent disulfide exchange. Pepsin or cyanogen bromide are particularly useful (see Chapters 76 and 71 respectively).
KeywordsCyanogen Bromide Iodoacetic Acid Peptide Bond Cleavage Reverse Phase HPLC Column Reductive Alkylation