Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis-Thin-Layer Chromatography

  • Ralph C. Judd
Part of the Springer Protocols Handbooks book series (SPH)


The principle behind peptide mapping is straightforward: If two proteins have the same primary structures, then cleavage of each protein with a specific protease or chemical cleavage reagent will yield identical peptide fragments. However, if the proteins have different primary structures, and then the cleavage will generate unrelated peptides. The similarity or dissimilarity of the proteins’ primary structure is reflected in the similarity or dissimilarity of the peptide fragments. Separation of peptides by 2-D thin-layer electrophoresis-thin-layer chromatography (2-D TLE-TLC) results in very high resolution of the peptides, making subtle comparisons possible. There are four phases to the 2-D TLE-TLC peptide mapping process:


Peptide Fragment Peptide Mapping Microfuge Tube Neutral Buffer Peptide Separation 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Ralph C. Judd
    • 1
  1. 1.Division of Biological ScienceUniversity of MontanaMissoula

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