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Pyridylethylation of Cysteine Residues

  • Malcolm Ward
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

To help maintain their three-dimensional structure, many proteins contain disulfide bridges between cysteine residues. Cysteine residues can cause problems during Edman sequence analysis, and quantification of cysteine and cystine by amino acid analysis is difficult since these residues are unstable during acid hydrolysis.

Keywords

Cysteine Residue Amino Acid Analysis Disulfide Bridge Laser Desorption Ionization Guanidine Hydrochloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Glazer, A. N., Delange, R. J., and Sigman, D. S. (1975) Chemical characterization of proteins and their derivatives, in Chemical Modifications of Proteins (Work, T. S and Work. E., eds.), North Holland, Amsterdam, 21–24.Google Scholar
  2. 2.
    Allen, G. (1989) Sequencing of proteins and peptides, in Laboratory Techniques in Biochemistry and Molecular Biology (Burdon, R. H. and Van Knippenberg, P. H., eds.), Elsevier, Amsterdam.Google Scholar
  3. 3.
    Vestling, M. M., Kelly, M. A., and Fenselau, C. (1994) Optimisation by mass spectrometry of a tryptophan-specific protein cleavage reaction. Rapid Commun. Mass Spectrom. 8, 786–790.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Malcolm Ward
    • 1
  1. 1.Proteome Sciences plcKings CollegeLondonUK

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