Utilization of Avidin or Streptavidin-Biotin as a Highly Sensitive Method to Stain Total Protein on Membranes

  • Kenneth E. Santora
  • Stefanie A. Nelson
  • Kristi A. Lewis
  • William J. LaRochelle
Part of the Springer Protocols Handbooks book series (SPH)


Since the initial publication by Towbin and coworkers (1) on the preparation of replicas of sodium dodecyl sulfate (SDS)-polyacrylamide gel patterns, commonly called protein blots, the technique of transferring proteins from inaccessible gel matrices to accessible solid supports, such as nitrocellulose or nylon membranes, has become widely utilized (2,3).


Sodium Dodecyl Sulfate Sodium Dodecyl Sulfate Horseradish Peroxidase Wash Solution Newborn Calf Serum 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Kenneth E. Santora
    • 1
  • Stefanie A. Nelson
    • 1
  • Kristi A. Lewis
    • 1
  • William J. LaRochelle
    • 1
  1. 1.Laboratory of Cellular and Molecular BiologyNational Cancer Institute, National Institute of HealthBethesda

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