Alkaline Phosphatase Labeling of IgG Antibody

  • G. Brian Wisdom
Part of the Springer Protocols Handbooks book series (SPH)


Alkaline phosphatase (EC from bovine intestinal mucosa is a valuable label. It is stable, has a moderate size (140 kDa), a high turnover number, and can be assayed using a variety of different substrates which are measured by changes in absorbance, fluorescence, or luminescence. The most common method of labeling immunoglobulin G (IgG) antibody with this enzyme uses the homobifunctional reagent glutaraldehyde.


Alkaline Phosphatase Free Thiol Group Short Glass Maleimide Group Transfer Loss 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • G. Brian Wisdom
    • 1
  1. 1.School of Biology and BiochemistryThe Queen’s University, Medical Biology CentreBelfastUK

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