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Radiolabeling of Eukaryotic Cells and Subsequent Preparation for 2-Dimensional Electrophoresis

  • Nick Bizios
Protocol
  • 97 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Two-dimensional polyacrylamide gel electrophoresis (2-DE) provides not only the ability to resolve and quantify thousands of proteins, but it also gives those in research and industry the ability to monitor in-process protein purification, quickly and easily N. Bizios, unpublished date, (AGI Dermatics). 2-DE is also used to identify variability in protein expression in a variety of cell lines (2,3). It is known that many parameters and laboratory conditions can influence the resolution of proteins on 2-DE, such as the pH range of carrier ampholytes used, the quality of reagents and equipment used, temperature, voltage, and the skill of the researcher or technician.

Keywords

Microcentrifuge Tube Tris Base Carrier Ampholyte Proper Documentation Dialyze Fetal Bovine Serum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    Carroll, K., Ray, K., Helm, B., and Carey, E. (2000) Two-dimensional electrophoresis reveals differential protein expression in high and low-secreting variants of the rat basophilic leukemia cell line. Electrophoresis 12, 2476–2486.CrossRefGoogle Scholar
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    Garrles, J. I. (1983) Quantitative two-dimensional gel electrophoresis of proteins, in Methods of Enzymology, Vol. 100 (Grossman, L., Moldave, K., and Wu, R., eds.), Academic Press, New York, pp. 411–423.Google Scholar
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    Garrels, J. I. and Franza, B. R.,Jr. (1989) The REF52 protein database. J. Biol. Chem. 264, 5283–5298.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Nick Bizios
    • 1
  1. 1.AGI DermaticsFreeport

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