Radiolabeling of Eukaryotic Cells and Subsequent Preparation for 2-Dimensional Electrophoresis
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Two-dimensional polyacrylamide gel electrophoresis (2-DE) provides not only the ability to resolve and quantify thousands of proteins, but it also gives those in research and industry the ability to monitor in-process protein purification, quickly and easily N. Bizios, unpublished date, (AGI Dermatics). 2-DE is also used to identify variability in protein expression in a variety of cell lines (2,3). It is known that many parameters and laboratory conditions can influence the resolution of proteins on 2-DE, such as the pH range of carrier ampholytes used, the quality of reagents and equipment used, temperature, voltage, and the skill of the researcher or technician.
KeywordsMicrocentrifuge Tube Tris Base Carrier Ampholyte Proper Documentation Dialyze Fetal Bovine Serum
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