Analysis of IgG Fractions by Electrophoresis
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After using a purification procedure it is necessary to obtain some index of purity obtained. One of the simplest methods for assessing purity of an IgG fraction is by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Although “full-size” slab gels can be used with discontinuous buffer systems and stacking gels, the use of a “minigel” procedure, using a Tris-bicine buffer system (1), rather than the classical Tris-glycine system, is quicker and easier, and gives improved resolution of immunoglobulin light-chains (these are usually smeared with the Tris-glycine system). Gel heights can be restricted to<10 cm and are perfectly adequate for assessing purity and monitoring column fractions.
KeywordsGlacial Acetic Acid Ammonium Persulfate Improve Resolution Relative Molecular Weight Discontinuous Buffer System
- 1.Johnstone, A. and Thorpe, R. (1996) Immunochemistry in Practice, 3rd ed., Blackwell Scientific, Oxford, UK.Google Scholar