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Analysis of IgG Fractions by Electrophoresis

  • Mark Page
  • Robin Thorpe
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

After using a purification procedure it is necessary to obtain some index of purity obtained. One of the simplest methods for assessing purity of an IgG fraction is by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Although “full-size” slab gels can be used with discontinuous buffer systems and stacking gels, the use of a “minigel” procedure, using a Tris-bicine buffer system (1), rather than the classical Tris-glycine system, is quicker and easier, and gives improved resolution of immunoglobulin light-chains (these are usually smeared with the Tris-glycine system). Gel heights can be restricted to<10 cm and are perfectly adequate for assessing purity and monitoring column fractions.

Keywords

Glacial Acetic Acid Ammonium Persulfate Improve Resolution Relative Molecular Weight Discontinuous Buffer System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Johnstone, A. and Thorpe, R. (1996) Immunochemistry in Practice, 3rd ed., Blackwell Scientific, Oxford, UK.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Mark Page
    • 1
  • Robin Thorpe
    • 2
  1. 1.Apovia Inc.San Diego
  2. 2.Division of ImmunobiologyNational Institute for Biological Standards and ControlPotters BarUK

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