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Use of Digoxigenin-Labeled Probes on Tissue Sections

  • Giorgio Terenghi
Protocol
  • 80 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Nonradioactively labeled probes offer several advantages compared to radioactive ones, as they show long stability, high morphological resolution, and rapid developing time. There are different types of nonradioactive labeling methods available, although digoxigenin-labeled probes (1) have become the most widely used for investigation on animal tissue, as they offer the advantage of low background noise and increased sensitivity (2,3). Also, digoxigenin can be used to label either RNA, DNA, or oligonucleotide probes. There have been different opinions on the sensitivity of detection of digoxigenin probes, but it has been shown that the sensitivity of radiolabeled and non-radioactive probes is comparable (3).

Keywords

Sodium Dodecyl Sulfate Acetic Anhydride cRNA Probe Reporter Molecule Target Nucleic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Giorgio Terenghi
    • 1
  1. 1.Blond McIndoe Laboratories, University Department of Surgery, Royal Free and University College Medical SchoolUniversity College LondonLondonUK

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