Use of Digoxigenin-Labeled Probes on Tissue Sections
- 80 Downloads
Nonradioactively labeled probes offer several advantages compared to radioactive ones, as they show long stability, high morphological resolution, and rapid developing time. There are different types of nonradioactive labeling methods available, although digoxigenin-labeled probes (1) have become the most widely used for investigation on animal tissue, as they offer the advantage of low background noise and increased sensitivity (2,3). Also, digoxigenin can be used to label either RNA, DNA, or oligonucleotide probes. There have been different opinions on the sensitivity of detection of digoxigenin probes, but it has been shown that the sensitivity of radiolabeled and non-radioactive probes is comparable (3).
KeywordsSodium Dodecyl Sulfate Acetic Anhydride cRNA Probe Reporter Molecule Target Nucleic Acid
- 5.Dirks, R. W., van Gijlswijk, R. P. M., Tullis, R. H., Smit, A. B., van Minnen, J., van der Ploeg, M., and Raap, A. K. (1990) Simultaneous detection of different mRNA sequences coding for neuropeptide hormones by double in situ hybridization using FITC-and biotin-labeled oligonucleotides. J. Histochem. Cytochem. 38, 467–473.PubMedCrossRefGoogle Scholar