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Primer-Directed Site-Specific Mutagenesis

  • Michael J. O’Donohue
  • G. Geoff Kneale
Protocol
  • 102 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Site-directed mutagenesis provides a powerful means for probing protein structure and function. Using this approach, one can introduce specific amino acid changes at any given position in the protein sequence and test the functional consequences of these mutations in vitro or in vivo. If an in vivo test is available, strategies that introduce random base changes at a given codon (or indeed in a larger region of the protein) are particularly valuable.

Keywords

Sodium Acetate Solution Ethidium Bromide Solution Primer Extension Reaction Mutagenic Oligonucleotide Mutagenesis Procedure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Michael J. O’Donohue
    • 1
  • G. Geoff Kneale
    • 2
  1. 1.UBPB-Fractionnement EnzymatiqueINRA-Lille/ReimsReimsFrance
  2. 2.Biophysics LaboratoriesUniversity of PortsmouthPortsmouthUK

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