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The Amplification Refractory Mutation System

  • John M. Old
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The amplification refractory mutation system (ARMS) is a simple and rapid method of detecting point mutations, restriction fragment length polymorphisms, and small deletions or insertions of a DNA sequence. The method was first described by Newton et al. (1) for the analysis of single nucleotide differences in DNA from patients with α − 1 antitrypsin deficiency and has since been applied for the carrier detection and prenatal diagnosis of many other genetics disorders including cystic fibrosis (2), β thalassaemia (3), and sickle cell disease (4).

Keywords

Cystic Fibrosis Amplification Refractory Mutation System Common Primer Ethidium Bromide Solution Antitrypsin Deficiency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Newton, C. R., Graham, A., and Heptinstall, L. E. (1989a) Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucl. Acids Res. 17, 2503–2516.PubMedCrossRefGoogle Scholar
  2. 2.
    Newton, C. R., Heptinstall, L. E., Summers, C., Super, M., Schwartz, M., Anwar, R., Graham, A., Smith, J. C., and Markham, A. F. (1989b) Amplification refractory mutation system for prenatal diagnosis and carrier assessment in cystic fibrosis. Lancet ii, 1481–1483.CrossRefGoogle Scholar
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    Old, J. M., Varawalla, N. Y., and Weatherall, D. J. (1990) The rapid detection and prenatal diagnosis of β thalassaemia in the Asian Indian and Cypriot populations in the UK. Lancet 336, 834–837.PubMedCrossRefGoogle Scholar
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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • John M. Old
    • 1
  1. 1.Institute of Molecular MedicineJohn Radcliffe HospitalHeadington, OxfordUK

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