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Mutation Screening Using PCR-SSCP

Silver Staining and Isotopic Protocols
  • Philip J. Saker
Protocol
  • 99 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Screening for mutations prior to sequencing can reduce the time and costs of identifying mutations. When the DNA sequence is known, the technique of detecting mutations as single-stranded conformational polymorphisms (SSCP) is a convenient method of screening for possible mutations. SSCP was originally developed by Orita et al. (1). It has the ability of detecting a single base change, and has been applied to a number of genes, including the insulin receptor (2), GLUT 4 (3), glucokinase (4), and the mitochondrial genome (5).

Keywords

Whatman Filter Paper Silver Stain Single Base Change Ammonium Persulphate Developer Solution 
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References

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Philip J. Saker
    • 1
  1. 1.Complex Traits Analysis Group, Imperial College School of MedicineHammersmith HospitalLondonUK

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